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Control of direct and indirect somatic embryogenesis by exogenous growth regulators in immature zygotic embryo cultures of rose

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Abstract

Immature zygotic embryos of rose (Rosa hybrida L.; cv. Sumpath) did not form somatic embryos or embryogenic calluses when cultured on half-strength Murashige and Skoog's medium supplemented with various con-centrations of 2,4-dichlorophenoxyacetic acid (2,4-D) as the sole growth regulator. However, the zygotic embryos produced somatic embryos without an intervening callus phase at a frequency of 27.3% on medium with 4.44 μM 6-benzyladenine (BA) alone. Immature zygotic embryos formed embryogenic calluses at a frequency of 25% on medium with a combination of 1.36 μM 2,4-D and 4.44 μM BA. Upon transfer to medium without growth regulators, embryogenic calluses produced numerous somatic embryos that subsequently developed into plantlets. Somatic embryos were induced directly from immature zygotic embryos, or indirectly via an intervening callus phase, by manipulating the exogenous growth regulators. Plantlets were successfully transplanted to potting soil and grown to maturity in a greenhouse.

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Authors and Affiliations

  1. KCTC, Genetic Resources Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), P.O. Box 115, Taejon, 305-600, South Korea

    Suk W. Kim

  2. Plant Cell Biotechnology Laboratory, Korea Research Institute of Bioscience and Biotechnology (KRIBB), P.O. Box 115, Taejon, 305-600, South Korea

    Seung C. Oh & Jang R. Liu

Authors
  1. Suk W. Kim
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  2. Seung C. Oh
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  3. Jang R. Liu
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Correspondence to Jang R. Liu.

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Kim, S.W., Oh, S.C. & Liu, J.R. Control of direct and indirect somatic embryogenesis by exogenous growth regulators in immature zygotic embryo cultures of rose. Plant Cell, Tissue and Organ Culture 74, 61–66 (2003). https://doi.org/10.1023/A:1023355729046

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  • DOI: https://doi.org/10.1023/A:1023355729046

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