Abstract
In capillary electrophoresis using poly(vinyl sulfate), added to sample solutionsand running buffers, the signal of Rhoda-mine 6G (R6G) is shaped like a squarewave. When α-, β-, or γ-cyclodextrin (α-, β-,or γ-CD) has further been added to the running buffers as well as samplesolutions, the intensity of the square-wave type signal of R6G has been enhanced,accompanied by narrowing of the signal width. The migration time of the risingedge of the square-wave type signal has been very slightly delayed by the additionof CD. These findings exhibit the formation of inclusion complexes of R6G withCDs. From the analyses of the variations in signal width with the concentration ofCD, the equilibrium constants for the formation of the 1:1 inclusion complexes ofR6G with α-, β-, and γ-CDs have been evaluated to be 245,210, and 302 M-1, respectively, although the equilibrium constants could not be estimated using a conventional procedure based on electrophoretic mobility, inwhich poly(vinyl sulfate) was not added. The values of the equilibrium constants forβ- and γ-CDs are comparable to those obtained from the absorbancechanges in spectrophotometry, whereas the value for α-CD is about four timesgreater than that obtained from the absorbance change.
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References
W. Saenger: Angew. Chem. Int. Ed. Engl. 19, 344 (1980).
S. Fanali: J. Chromatogr. A 875, 89 (2000).
A.M. Stalcup and K.H. Gahm: Anal. Chem. 68, 1360 (1996).
D.W. Armstrong, L.W. Chang, and S.S.C. Chang: J. Chromatogr. A 793, 115 (1998).
W. Zhu, F Wu, F.M. Raushel, and G. Vigh: J. Chromatogr. A 895, 247 (2000).
D.M. Davies and M.E. Deary: J. Chem. Soc., Perkin Trans. 2 2423 (1996).
C.N. Sanrame, R.H. de Rossi, and G.A. Arguello: J. Phys. Chem. 100, 8151 (1996).
S. Hamai and H. Sakurai: J. Chromatogr. A 800, 327 (1998); S. Hamai and H. Sakurai: Anal. Chim. Acta 402, 53 (1999).
S. Hamai: J. Phys. Chem. B 101, 1707 (1997).
S. Hamai: J. Phys. Chem. B 103, 293 (1999).
O.S. Tee, S.M.I. Hussein, I.E. Turner, and O.J. Yazbeck: Can. J. Chem. 78, 436 (2000).
S. Hamai and K. Sasaki: Microchem. J. 69, 27 (2001).
K.L. Rundlett and D.W. Armstrong: J. Chromatogr. A 721, 173 (1996).
B.A. Williams and G. Vigh: Anal. Chem. 68, 1174 (1996).
S. Hamai: Bull. Chem. Soc. Jpn. 55, 2721 (1982).
A. Harada: Coordinat. Chem. Rev. 148, 115 (1996).
J.P. Quirino and S. Terabe: Anal. Chem. 71, 1638 (1999).
H.A. Benesi and J.H. Hildebrand: J. Am. Chem. Soc. 71, 2703 (1949).
L. Flamigni: J. Phys. Chem. 97, 9566 (1993).
Y. Degani, I. Willner, and Y. Haas: Chem. Phys. Lett. 104, 496 (1984).
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Hamai, S., Sasaki, K. Investigation of the Formation of Inclusion Complexes between Rhodamine 6G and Cyclodextrins by Means of Capillary Electrophoresis Using Poly(vinyl sulfate. Journal of Inclusion Phenomena 45, 19–25 (2003). https://doi.org/10.1023/A:1023089809265
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DOI: https://doi.org/10.1023/A:1023089809265