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Casein hydrolysis in PEG-salt two phase system by Eudragit-chymotrypsin bioconjugate

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Abstract

A bioconjugate of α-chymotrypsin and Eudragit S-100 was used in an aqueous two-phase system (polyethylene glycol/phosphate) for casein hydrolysis. More product was obtained by replacing the lower salt phase with a fresh one during the reaction. The bioconjugate could be reused six times for casein hydrolysis.

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Authors and Affiliations

  1. Chemistry Department, Indian Institute of Technology, Delhi, Hauz Khas, New Delhi, 110 016, India

    Sunita Teotia, Shweta Sharma & M.N. Gupta

Authors
  1. Sunita Teotia
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  2. Shweta Sharma
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  3. M.N. Gupta
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Teotia, S., Sharma, S. & Gupta, M. Casein hydrolysis in PEG-salt two phase system by Eudragit-chymotrypsin bioconjugate. Biotechnology Letters 25, 637–639 (2003). https://doi.org/10.1023/A:1023064131466

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