Abstract
Time-resolved anisotropy measurements were performed using simple instrumentation with the aim to demonstrate the speed and ease of the experiment. Subsequent data analysis examples involved common, easily adaptable procedures.
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REFERENCES
M. Wahl and R. Erdmann (2000) Time-Correlated Single Photon Counting in Fluorescence Lifetime Analysis, Photonik 1–2/2000 (reprint available from PicoQuant, Berlin, Germany).
http://www.picoquant.com/_systems.htm.
M. Wahl, U. Ortmann, K. Lauritsen, and R. Erdmann (2002) in: A. K. Chin, N. K. Dutta, R. W. Herrick, K. J. Linden, D. J. McGraw (Eds.), Proceedings of SPIE Vol.4648, Test and Measurement Applications of Optoelectronic Devices, SPIE-The International Society for Optical Engineering, Bellingham, Wa, pp. 171–178.
M. Böhmer, F. Pampaloni, M. Wahl, H.-J. Rahn, R. Erdmann, and J. Enderlein (2001) Time-resolved confocal scanning device for ultrasensitive fluorescence detection. Rev. Sci. Instrum. 72(11), 4145–4152.
J. R. Lakowicz (1999) Principles of Fluorescence Spectroscopy 2nd edn., Kluwer Academic/Plenum Publishers, New York.
J. R. Lakowicz (Ed.)(1991) Topics in Fluorescence Spectroscopy, Vols. 1 and 2, Plenum Press, New York.
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Kapusta, P., Erdmann, R., Ortmann, U. et al. Time-Resolved Fluorescence Anisotropy Measurements Made Simple. Journal of Fluorescence 13, 179–183 (2003). https://doi.org/10.1023/A:1022995327718
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DOI: https://doi.org/10.1023/A:1022995327718