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RFLP analysis of mitochondrial DNA in eggplant and related Solanum species

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Abstract

RFLP analysis of mitochondrial DNA (mtDNA) was performed by the Southern hybridization method using total DNA extracted from eggplant, Solanum melongena L., and six related Solanum species, S. surattense Burm. (i.e. S. virginianum L.), S. torvum Swartz, S. gilo Raddi (i.e. S. aethiopicum), S. integrifolium Poir. (i.e. S. aethiopicum), S. indicum auct. non L. (i.e. S. violaceum Ort.) and S. sanitwongsei Craib. Forty-one fragments were detected by the analysis using 12 combinations of four restriction enzymes and three probes of mtDNA clones from rice. Thirty-four out of the 41 fragments were polymorphic among the species, whereas the other seven were monomorphic. This RFLP analysis of mtDNA is demonstrated to be appropriate for assessing phylogenetic relationships in eggplant and related Solanum species at the interspecific level.

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Authors and Affiliations

  1. Department of Biotechnology and Plant Breeding, Faculty of Agriculture, Saga University, Saga, 840-8502, Japan

    Shiro Isshiki, Shinji Suzuki & Ken-ichiro Yamashita

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  1. Shiro Isshiki
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  2. Shinji Suzuki
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  3. Ken-ichiro Yamashita
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Isshiki, S., Suzuki, S. & Yamashita, Ki. RFLP analysis of mitochondrial DNA in eggplant and related Solanum species. Genetic Resources and Crop Evolution 50, 133–137 (2003). https://doi.org/10.1023/A:1022954229295

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