Abstract
In vitro bulblet formation was studied using solid, liquid and bioreactor culture (immersion and periodic immersion in liquid media using ebb and flood) in order to develop a cost effective method for the mass propagation of Lilium oriental hybrid ‘Casablanca’. Although the percent of bulblet formation was higher in solid culture, the increased growth rate and production of large number of bulblets in bioreactor makes it suitable for mass propagation. Four times per day and 15 min of medium supply was optimal for bulblet formation in ebb and flood bioreactor. Bulblet formation was also found to be effective in 16-h photoperiod. It was also observed that bulblet formation in the medium with 1.0 mg dm−3 BA and 0.3 mg dm−3 NAA was higher than in the medium without growth regulators, but formation of abnormal bulblets was higher in medium with BA and NAA.
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Lian, M., Chakrabarty, D. & Paek, K. Bulblet Formation from Bulbscale Segments of Lilium Using Bioreactor System. Biologia Plantarum 46, 199–203 (2003). https://doi.org/10.1023/A:1022890208500
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DOI: https://doi.org/10.1023/A:1022890208500