Skip to main content
SpringerLink
Log in

Effect of inoculation with ecto- and arbuscular mycorrhizae and Rhizobium on the growth and nitrogen fixation by black locust, Robinia pseudoacacia

  • Published:
New Forests Aims and scope Submit manuscript

Abstract

Experiments were made to determine the effect of inoculation withRhizobium, the ectomycorrhizal fungus Hebelomamesophasem and the arbuscular mycorrhizal fungus Glomuscaledonium on the growth and nitrogen fixation of black locust(Robinia pseudoacacia Linn.) seedlings grown invitro and in pot culture. The results showed that both mycorrhizalfungi and Rhizobium stimulated the growth and nitrogenfixing ability of inoculated seedlings. Inoculation with all three microbestogether produced the most beneficial effects on nitrogen fixation, mycorrhizaldevelopment and seedling growth.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bi J., Gao H.Z. and Wang Z.L. 1995. Current situation and trend of black locust researches. J. Hebei Forestry College 10 (in Chinese): 92–95.

    Google Scholar 

  • Biro B., Koves-Pechy K., Voros I., Takacs T., Eggenberger P. and Strasser R.J. 2000. Interrelations between Azospirillum and Rhizobium nitrogen-fixers and arbuscular mycorrhizal fungi in rhizosphere of alfalfa in sterile, AMF-free or normal soil conditions. Appl. Soil Ecol. 15: 159–168.

    Google Scholar 

  • Biro B., Voros I., Koves-Pechy K. and Szegi J. 1993. Symbiont effect of Rhizobium bacteria and AM fungi on Pisum sativum in recultivated mine spoils. Geomicrobiol. J. 11: 275–284.

    Google Scholar 

  • Chanway C.P. 1997. Inoculation of tree roots with plant growth promoting soil bacteria: An emerging technology for reforestation. Forest Sci. 41: 99–112.

    Google Scholar 

  • Chatarpaul L., Chakravarty P. and Subramaniam P. 1989. Studies in tetrapartite symbiosis 1: Role of ecto-and endomycorrhizal fungi and Frankia on the growth performance of Alnus incana. Pl. Soil. 118: 145–150.

    Google Scholar 

  • Frioni L., Minasian H. and Volfovicz R. 1999. Arbuscular mycorrhizae and ectomycorrhizae in native tree legumes in Uruguay. Forest Ecol. Manag. 115: 41–47.

    Google Scholar 

  • Hardy R.W.F., Bums R.C. and Hosten R.D. 1973. Application of the acetylene-ethylene assay for measurement of nitrogen fixation. Soil Biol. Biochem. 5: 7–81.

    Google Scholar 

  • Hayman D.S., Johnson A.M. and Dusslesdin I. 1975. The influence of phosphate and crop species on endogome spores and vesicular-arbuscular mycorrhiza under field conditions. Pl. Soil. 43: 489–495.

    Google Scholar 

  • Jha D.K., Sharma G.D. and Mishra R.R. 1993. Mineral nutrition in the tripartite interaction between Frankia, Glomus and Alnus at different soil phosphorus regimes. New Phytol. 123: 307–311.

    Google Scholar 

  • Malváarez G., Major G., Curbelo V. and Frioni L. 1997. Hongos ectomicorricicos en Eucalyptus grandis. Agrociencia, Facultad de Agronomia. Montevideo 1: 38–43.

    Google Scholar 

  • Olesniewicz K.S. and Thomas R.B. 1999. Effects of mycorrhizal colonization on biomass production and nitrogen fixation of black locust (Robinia pseudoacacia) seedlings grown under elevated atmospheric carbon dioxide. New Phytol. 142: 133–140.

    Google Scholar 

  • Pacovsky R.S., Fuller G. and Paul E.A. 1985. Influence of soil on the interactions between endomycorrhizae and Azospirillum on sorghum. Soil Biol. Biochem. 17: 521–531.

    Google Scholar 

  • Phillips J.M. and Hayman D.S. 1970. Improved procedures for clearing and attaining parasitic and vesicular-arbuscular mycorrhizal fungi for rapid assessment of infection. Trans. Br. Mycol. Soc. 55: 158–161.

    Google Scholar 

  • Raju P.S., Clark R.B. and Ellis J.R. 1990. Effects of species of VAM fungi on growth and mineral uptake of sorghum at different temperatures. Pl. Soil. 121: 165–170.

    Google Scholar 

  • Rao A.V. and Tak R. 2001. Influence of mycorrhizal fungi on the growth of different tree species and their nutrient uptake in gypsum mine spoil in India. Appl. Soil Ecol. 17: 279–284.

    Google Scholar 

  • Sanchez-Diaz M., Pardo M., Antolin M., Pena J. and Aguirreola J. 1990. Effect of water stress onphotosynthetic activity in theMedicago-Rhizobium-Glomus symbiosis. Plant Sci. 71: 215–221.

    Google Scholar 

  • Sprent J.I. and Parsons R. 2000. Nitrogen fixation in legume and non-legume trees. Field Crop. Res. 65: 183–196.

    Google Scholar 

  • Shanghai Society of Plant Physiology 1985. Handbook for Plant Physiology. Shanghai Science and Technology Press, Shanghai, (In Chinese).

    Google Scholar 

  • Tian C.J., He X.Y., Han G.Y. and Wu Q.F. 2000. Investigation, isolation and inoculation of mycorrhizae for Robinia pseudoacacia. Chinese J. Ecol. 19 (in Chinese): 16–20.

    Google Scholar 

  • Tian C.J., He X.Y., Zhong Y. and Chen J.K. 2002. Effects of VA mycorrhizae and Frankia dual inoculation on growth and nitrogen fixation of Hippophae thibetana. Forest Ecol. Manag. MS Code: FORECO5822. (in press).

  • Trouvelot A., Kough J.L. and Gianinazzi-Pearson V. 1985. Mesure du Taux de mycorrhization VA d'un systeme radiculaire Symposium Europeen Sur Les Mycorrhizaes, INRA, Paris., pp. 217–221

    Google Scholar 

  • Vincent J.M. 1970. A manual for the practical study of the root-nodule bacteria. IBP Handbook No15. Blackwell Scientific Publications, Oxford.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Biodiversity Science, Fudan University, Ministry of Education Key Laboratory for Biodiversity Science and Ecological Engineering, Shanghai, 200433, The People's Republic of China

    Chunjie Tian, Yang Zhong & Jiakuan Chen

  2. Academia Sinica, Shenyang Institute of Applied Ecology, Shenyang, 110015, The People's Republic of China

    Xingyuan He

Authors
  1. Chunjie Tian
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Xingyuan He
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yang Zhong
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jiakuan Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Chunjie Tian.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tian, C., He, X., Zhong, Y. et al. Effect of inoculation with ecto- and arbuscular mycorrhizae and Rhizobium on the growth and nitrogen fixation by black locust, Robinia pseudoacacia . New Forests 25, 125–131 (2003). https://doi.org/10.1023/A:1022675915627

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1022675915627

Search

Navigation