Abstract
Grape seeds were used by Trametes hirsuta as a substrate for laccase production giving 23 kU l−1, which was 10-fold the value attained in the cultures with no lignocellulosic waste addition. The dyes, Indigo Carmine and Bromophenol Blue, were easily decolourised (100% in 24 h) by the extracellular liquid obtained in such cultures, whereas Methyl Orange (65% in 24 h) and Phenol Red (36% in 24 h) were more resistant to degradation. This shows the specificity of laccase towards different dye structures.
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Moldes, D., Gallego, P., Rodríguez Couto, S. et al. Grape seeds: the best lignocellulosic waste to produce laccase by solid state cultures of Trametes hirsuta . Biotechnology Letters 25, 491–495 (2003). https://doi.org/10.1023/A:1022660230653
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DOI: https://doi.org/10.1023/A:1022660230653