Abstract
Increasing demand for ferns means that more knowledge of propagation techniques be developed to guarantee the continuous supply of these plants from commercial ornamental companies. In vitro tissue culture techniques show a certain promise with respect to a future increase in production. This review summarises all the research that has been conducted with ornamental ferns using in vitro techniques at different phases in their life cycle, starting from spores, to gametophytes and sporophytes. Nutritional, environmental and other factors affecting growth and development in each phase of the life cycle of the fern are discussed, highlighting some protocols that enhance their morphogenic and reproductive capacity.
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References
Amaki W & Higuchi H (1991) A possible propagation system of Nephrolepis, Asplenium, Pteris, Adiantum and Rhumora through tissue culture. Acta Hort. 300: 237-243
Beck MJ & Caponetti JD (1983) The effects of kinetin and naphtaleneacetic acid in vitro shoot multiplication and rooting in the fishtail fern. Am. J. Bot. 70: 1-7
Bell PD (1959) The experimental investigation of the pteridophyte life cycle. J. Linn. Soc. Bot. London 56: 188-202
Bertrand AM, Albuerne MA, Fernandez H, Gonzalez A & Sanchez-´-Tames R (1999) In vitro organogenesis of Polypodium cam-bricum. Plant Cell Tiss. Org. Cult. 57: 65-69
Bopp M (1968) Control of differentiation in fern allies and bryophytes. Ann. Rev. Plant Physiol. 19: 361-380
Cooke RC (1979) Homogenization as an aid in tissue culture propagation of Platycerium and Davallia. HortSci. 14: 21-22
Corey EJ, Myers AG, Takahashi N, Yamane H & Schraudolf H (1986) Constitution of antheridium-inducing factor of Anemia phyllitidis. Tetrahedron. Lett. 27: 5083-5084
Cousens MI (1979) Gametophytic ontogeny, sex expression and genetic load as measures of population divergence in Blechnum spicant. Am. J. Bot. 66: 116-132
Deberg P (1994) In vitro culture of ornamentals. In: Vasil IK & Thorpe TA (eds) Plant Cell and Tissue Culture (pp. 561-573). Kluwer Academic Publishers, Dordrecht, The Netherlands
Dopp W (1950) Eine die Antheridienbildung bei farnen fordernde substanz in den prothallien von Pteridium aquilinum (L.) Kuhn. Ver Deut. Bot. Ges. 63: 139
Douglas GE & Sheffield E (1992) The investigation of existing and novel artificial growth systems for the production of fern gametophytes. In: Ide JM, Jermy CA & Paul AM (eds) Fern Horticulture: Past. Present and Future Perspectives (pp. 183- 187). Intercept, Andover
Dyer AF (1979) The culture of fern gametophytes for experimental investigation. In: Dyer AF (ed) The Experimental Biology of Ferns (pp. 253-305). Academic Press, London
Emigh VD & Farrar DR (1977) Gemmae: a role in sexual reproduc-tion in the fern genus Vittaria. Science 198: 297-298
Farrar DR (1974) Gemmiferous fern gametophytes Vittariaceae. Am. J. Bot. 61: 146-155
Farrar DR & Johnson-Groh CL (1990) Subterranean sporophytic gemmae in moowort ferns, Botrychium subgenus Botrychium. Am. J. Bot. 77: 1168-1175
Fay MF (1994) In what situations is in vitro culture appropriate to plant conservation? Biodiversity and Conservation 3: 176-183
Fernández H, Bertrand AM & Sanchez Tames R (1993) In vitro regeneration of Asplenium nidus L. from gametophytic and sporophytic tissue. Sci. Hort. 56: 71-77
Fernández H, Bertrand AM & Sánchez-Tamés R (1996a) Influence of tissue culture conditions on apogamy in Dryopteris affinis sp. affinis. Plant Cell Tiss. Org. Cult. 45: 93-97
Fernández H, Bertrand AM & Sánchez-Tamés R (1996b) Micro-propagation and phase change in Blechnum spicant and Pteris ensiformis. Plant Cell Tiss. Org. Cult. 44: 261-265
Fernández H, Bertrand AM & Sánchez-Tamés R (1997a) Gemma-tion in Osmunda regalis L. gametophyte cultured in vitro. Plant Cell Rep. 16: 358-362
Fernández H, Bertrand AM & Sánchez-Tamés R (1997b) Plantlet regeneration in Asplenium nidus L. and Pteris ensiformis L. by homogenisation of BA treated rhizomes. Sci. Hort. 68: 243-247
Fernández H, Bertrand AM, Feito I & Sánchez-Tamés R (1997c) Gametophyte culture in vitro and antheridiogen activity in Blech-num spicant. Plant Cell Tiss. Org. Cult. 50: 71-74
Fernández H, Doumas P & Masimbert M (1997d) Quantification of GA1, GA3, GA4, GA7, GA9, GA19 and GA20, and GA20 metabo-lism in dormant and non-dormant beechnuts. Plant Growth Reg. 22: 29-35
Fernández H, Bertrand AM & Sánchez-Tamés R (1999a) Bio-logical and nutritional aspects involved in fern multiplication. Plant Cell Tiss. Org. Cult. 56: 211-214
Fernández H, Bertrand AM, Sierra MI & Sánchez-Tamés R (1999b) An apolar GA-like compound responsible for the antheridiogen activity in Blechnum spicant. Plant Growth Reg. 28: 143-144
Finnie JF & van Staden J (1987) Multiplication of the tree fern Cyathea degrei. HortSci. 22: 665
Harper K (1976) Asexual Multiplication of Leptosporangiatae Ferns Through Tissue Culture. MSC Thesis. University of Cali-fornia, Riverside (pp. 168)
Hedge S (1998) Propagation of Some Native Ornamental Plants using Biotechnology, Ph.D Thesis, Mangalore University, Mangalore, India
Hedge S & D'Souza L (2000) Recent advances in biotechnology of ferns. In: Trivedi PC (ed) Plant Technology,. Panima Publishing Corporation, New Delhi, Bangalore
Hicks G & von Aderkas P (1986) A tissue culture of the ostrich fern Matteuccia struthiopteris L. Todaro. Plant Cell Tiss. Org. Cult. 5: 199-204
Higuchi H & Amaki W(1989) Effects of 6-benzylaminopurine on the organogenesis of Asplenium nidus L. through in vitro propa-gation. Sci. Hort. 37: 351-359
Higuchi H, Amaki W & Suzuki S (1987) In vitro propagation of Nephrolepis cordiflora. Sci. Hort. 32: 105-113
Hotta Y & Osawa S (1958) Control of differentiation in the fern gametophyte by amino acid analogs and 8-azaguanine. Exp. Cell Res. 15: 85-94
Hughes SJ (1971) On conidia of fungi and gemmae of algae, bryophytes and pteridophytes. Can. J. Bot. 49: 1319-1339
Kato Y (1964) Physiological and morphogenetic studies of fern gametophytes in aseptic culture II. One and two-dimensional growth in sugar media. Bot. Gaz. 125: 33-37
Kaur S (1991) Ferns and fern allies. Their domestication and conservation. In: Bharadwaj TN & Gena CB (eds) Perspectives in Pteridology: Present and Future. Today & Tomorrow's Prin-ters and Publishers, New Delhi (India), Vol. 13 (pp. 83-89). Aspects of Plant Sciences.
Klekowski EJ Jr. (1969) Reproductive biology of the Pteridophyta. II. Theoretical considerations. J. Linn. Soc. (Bot.). 62: 347-359
Klekowski EJ Jr. (1973) Genetic load in Osmunda regalis popula-tions. Am. J. Bot. 60: 146-154
Klekowski EJ Jr. & Lloyd RM (1968) Reproductive biology of the Pteridophyta I. General considerations and a study of Onoclea sensibilis L. J. Linn. Soc. (Bot.) 60: 315-324
Knauss JF (1976) A partial tissue culture method for pathogen-free propagation of selected ferns from spores. Proc. Fla State Hort. Soc. 89: 363-365
Knop W (1865) Quantitative untersuchungen uber die er-nahrungsprozesse der pflanzen. LandwirtschVers Stn. 7: 93-107
Knudson L (1946) A nutrient solution for the germination of orchid seed. Bull. Am. Orchid Soc. 15: 214-217
Kuriyama A, Sugawara Y, Matsushima H & Takeuchi M (1990) Production of sporophytic structures from gametophytes by cytokinin in Equisetum arvense. Naturwissenschften 77: 31-32
Linsay J (1994) Account of germination and raising of ferns from the seed. Trans. Linn. Soc. London 2: 93-100
Loescher WH & Albrecht CN (1979) Development in vitro of Nephrolepis exaltata ''Bostoniensis' runner tissue. Physiol. Plant 47: 250-254
Melan MA & Whittier DP (1990) Effects of inorganic nitrogen sources on spore germination and gametophyte growth in Botrychium dissectum. Plant Cell Environ. 13: 477-482
Miller JH (1968) Fern gametophytes as experimental material. Bot. Rev. 34: 361-440
Mohr H (1962) The influence of visible radiation on the germina-tion of archegoniate spores and the growth of the fern protonema. J. Linn. Soc. Bot. 58: 287-296
Murashige T (1974) Current status of plant cell and organ culture. Hort. Sci. 12: 3-6
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant 15: 473-497
Näf U (1979) Antheridiogens and antheridial development. In: Dyer AF (ed) Exp Biol Ferns,. Academic Press, London ¨
Näf U, Sullivan J & Cummins M (1969) New antheridiogen from the fern Onocleasensibilis. Science 163: 1357
Peck JH, Peck CJ & Farrar DR (1990) Influences of life history attributes on formation of local and distant fern populations. Am. Fern. J. 80: 126-142
Pierik RLM (1991) Commercial aspects of micropropagation. In: Prakash J & Pierik RML (eds) Horticulture - New Technologies and Applications (pp. 141-153). Kluwer Academic Publishers, Dordrecht, The Netherlands
Pierik RML, Oerlemans APC, Verhoeven MAP, Mentjox MA & Molenberg H (1986) DeZaai van varens in kweekbuizen. Vakblad Bloemisterij 10: 48-49
Sheffield E & Bell PR (1987) Current studies of the pteridophyte life cycle. Bot. Rev. 53: 442-490
Sheffield E & Farrar DR (1988) Cryo SEM examination of gemma formation in Vittaria raminifolia. Am. J. Bot. 75: 894-899
Skoog F & Miller CO (1957) Chemical regulation of growth and organ formation in plant tissues cultured in vitro. Proc. Symp. Soc. Exp. Biol. 11: 118-131
Smith CW & Yee RNS (1975) The effect of coconut milk on the germination and growth of spores of Nephrolepis hirsutula. Am. Fern. J. 65: 13-18
Swami P & Raghavan V (1980) Control of morphogenesis in the gametophyte of a fern by light and growth hormones. Can. J. Bot. 58: 1464-1473
Whittier DP (1962) The origin and development of apogamous structures in the gametophyte of Pteridium in sterile culture. Phytomorphology 12: 11-20
Whittier DP (1965) Obligate apogamy in Cheilantes tomentosa & C. alabamiensis. Bot. Gaz. 126: 275-281
Whittier DP (1966) The influence of growth substances on the induction of apogamy in Pteridium gametophytes. Am. J. Bot. 53: 882-886
Whittier DP (1975) The influence of osmotic conditions on induced apogamy in Pteridium gametophytes. Phytomorphology 25: 246-249
Whittier DP (1981) Spore germination and young gametophyte development of Botrychium and Ophioglossum in actinic culture. Am. Fern. J. 71: 13-19
Whittier DP (1989) Effects of nitrogen source on spore germination and gametophyte growth in Psilotum. Bot. Gaz. 151: 50-53
Whittier DP & Steeves TA (1960) The induction of apogamy in the bracken fern. Can. J. Bot. 38: 925-930
Whittier DP & Steeves TA (1962) Further studies on induced apogamy in ferns. Can. J. Bot. 40: 1525-1531
Yamane H, Takahashi N, Takeno K & Furuya M (1979) Identific-tion of gibberellin A methyl ester as a natural substance regulat-ing formation of reproductive organs in Lygodium japonicum. Planta 147: 251-256
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Fernández, H., Revilla, M. In vitro culture of ornamental ferns. Plant Cell, Tissue and Organ Culture 73, 1–13 (2003). https://doi.org/10.1023/A:1022650701341
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DOI: https://doi.org/10.1023/A:1022650701341