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Responses of strawberry plantlets cultured in vitro under superbright red and blue light-emitting diodes (LEDs)

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Abstract

Unrooted strawberry cv. `Akihime' shoots with three leaves obtained from standard mixotrophic cultures were cultured in the ``Culture Pack''-rockwool system with sugar-free MS medium under CO2-enriched condition. To examine the effect of superbright red and blue light-emitting diodes (LEDs) on in vitro growth of plantlets, these cultures were placed in an incubator, ``LED PACK'', with either red LEDs, red LEDs1blue LEDs or blue LEDs light source. To clarify the optimum blue and red LED ratio, cultures were placed in ``LED PACK 3'' under LED light source with either 100, 90, 80, or 70% red + 0, 10, 20, 30% blue, respectively, and also under standard heterotrophic conditions. To determine the effects of irradiation level, cultures were grown under 90% red LEDs + 10% blue LEDs at 45, 60 or 75 μmol m−2 s−1 . Plantlet growth was best at 70% red + 30% blue LEDs. The optimal light intensity was 60 μmol m−2 s−1. Growth after transfer to soil was also best after in vitro culture with plantlets produced were 70% red LEDs + 30% blue LEDs.

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Authors and Affiliations

  1. Faculty of Agriculture, Kagawa University, Miki-cho, Kagawa, 761-0795, Japan

    Duong Tan Nhut, T. Takamura & M. Tanaka

  2. Yokohama Research Center, Mitsubishi Chemical Corp., Yokohama, 227-8502, Japan

    H. Watanabe

  3. Faculty of Engineering, Kagawa University, Hayashi-cho, Takamatsu, 761-0396, Japan

    K. Okamoto

Authors
  1. Duong Tan Nhut
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  2. T. Takamura
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  3. H. Watanabe
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  4. K. Okamoto
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  5. M. Tanaka
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Correspondence to M. Tanaka.

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Nhut, D.T., Takamura, T., Watanabe, H. et al. Responses of strawberry plantlets cultured in vitro under superbright red and blue light-emitting diodes (LEDs). Plant Cell, Tissue and Organ Culture 73, 43–52 (2003). https://doi.org/10.1023/A:1022638508007

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