Abstract
We have isolated a homolog of the Arabidopsisthaliana IAA amidohydrolase ILR1 from the related speciesArabidopsis suecica. Employing PCR primers targeted to the5′ and 3′ untranslated regions of ILR1, A.suecica produced an amplified, genomic product (sILR1) of 2027 bp. Thecloned sILR1 gene was sequenced, and the DNA homology between sILR1 and ILR1found to be 98%. The sILR1 cDNA was isolated using RT-PCR, cloned and sequenced.The sILR1 cDNA was found to have 98% homology to its homolog ILR1 both at theDNA and amino acid level. The sILR1 mRNA transcript was found to be expressed inA. suecica tissues at ages 5, 10, and 15 days.Additionally, primers, designed to a ∼300 bp domain conserved in bacterial andplant amidohydrolases, were used to amplify a product from seven members of theBrassicaceae family (A. suecica, A. pumila, A.himalaica, A. griffithiana, A. wallichii, Draba nemoroza andCapsella bursa-pastoris), Lycopersiconesculentum, and two monocot species (Zea maysand Allium cepa) using PCR; this result suggests similargenes may be characterized in other species.
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Campanella, J.J., Bakllamaja, V., Restieri, T. et al. Isolation of an ILR1 auxin conjugate hydrolase homolog from Arabidopsis suecica . Plant Growth Regulation 39, 175–181 (2003). https://doi.org/10.1023/A:1022528318188
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DOI: https://doi.org/10.1023/A:1022528318188