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Small-Angle Neutron Scattering Studies of Vesicle Stability

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Abstract

Small-angle neutron scattering (SANS) was used to investigate the structure of mixed colloids of egg yolk phosphatidylcholine (EYPC) with the bile salt, cholylglycine (CG), in D2O as a function of pressure (P) and temperature (T). At atmospheric pressure, the system forms an isotropic phase of mixed, single-bilayer vesicles (SLVs). Increasing the external hydrostatic pressure brought about significant changes in particle morphology. At T=25°C, application of a pressure of 3.5 MPa resulted in collapse of the SLVs. A further increase in P, up to 51.8 MPa, resulted in a transition from a phase of ordered (stacked), collapsed vesicles to one of stacked, ribbon-like particles. A similar collapse of the vesicles was observed at a higher temperature (T=37°C) with increasing P, but at this temperature, no ribbon phase was found at the highest pressure explored.

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Authors
  1. J. T. Mang
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  2. R. P. Hjelm
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Mang, J.T., Hjelm, R.P. Small-Angle Neutron Scattering Studies of Vesicle Stability. International Journal of Thermophysics 19, 461–470 (1998). https://doi.org/10.1023/A:1022517511951

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  • DOI: https://doi.org/10.1023/A:1022517511951

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