Abstract
Research on the effects of glyphosate and seed germination spans 30 years. Despite several studies reporting detrimental effects of the herbicide on seedling germination and growth, glyphosate is still being registered for use as a weed killer and preharvest desiccant. Its nonselective nature and low chance of species developing resistance has lead to the development of genetically modified crops tolerant to the herbicide which also raises concerns about increased reliance on herbicide use, and subtle ecological impact.
This paper presents the result of a literature review on past studies mostly, on crop species, and the results of a new experiment performed with emphasis on noncrop species. The new experimental part attempted to determine whether glyphosate (Roundup®) would have an effect on the germination and growth of the F1 generation of seeds produced by plants sprayed with the herbicide. It was designed to mirror spray drift which might affect noncrop plants in nontarget drift zones. Of the 11 species tested using treatments of 0% (control), 1%, 10% or 100% of a 890 g a.i./ha label rate solution sprayed near seed maturity, seven showed a significant effect of the glyphosate treatment on germination and/or growth characteristics.
Results of this experiment together with several previous studies reviewed in this paper suggest that there are significant effects to keep in mind when using herbicides such as glyphosate as severe ecological changes could occur.
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References
Agriculture Canada (Food Production and Inspection Branch, Pesticides Directorate, Pesticides Information Division) (1991). Pre-harvest Use of Glyphosate Herbicide: Discussion Document.
Anonymous (1995). Seed industry concerned about poor germination rates. Pest Manag. News 7(2), 7.
Arnaud, L., Nurit, F., Ravanel, P. and Tissut, M. (1994). Distribution of glyphosate and of its target enzyme inside wheat plants. Pestic. Sci. 40, 217-23.
Azlin, W.R. and McWhorter, C.G. (1981). Preharvest effects of applying glyphosate to soybeans (Glycine max). Weed Sci. 29(1), 123-7.
Baskin, C.C. and Baskin, J.M. (1998). Seeds: Ecology, Biogeography, and Evolution of Dormancy and Germination. USA: Academic Press.
Baur, J.R., Miller, F.R. and Bovey, R.W. (1977). Effects of preharvest desiccation with glyphosate on grain sorghum seed. Agronomy J. 69, 1015-18.
Baylis, A.D. (2000). Why glyphosate is a global herbicide: strengths, weaknesses and prospects. Pest Manag. Sci. 56, 299-308.
Bennett, A.C. and Shaw, D.R. (2000). Effect of preharvest desiccants on Group IV glycine max seed viability. Weed Sci. 48, 426-30.
Bovey, R.W., Miller, F.R. and Baur, J.R. (1975). Preharvest desiccation of grain sorghum with glyphosate. Agron. J. 67, 618-21.
Buhler, D.D. and Burnside, O.C. (1987). Effects of application variables on glyphosate phytotoxicity. Weed Technol. 1, 14-17.
Campbell, M.H., Miller, L.G. and Nicol, H.I. (1998). Effect of herbicides on seedhead production and control of serrated tussock (Nassella trichotoma (Nees) Arech.). Plant Prot. Quart. 13(3), 106-10.
Canadian Food Inspection Agency, (2001). Glyphosate tolerant crops (Monsanto Canada). www.cfia-acia.agr.ca/english/plaveg/pbo/pntvcne.shtml.
Carlisle, S.M. and Trevors, J.T. (1988). Glyphosate in the environment. Water. Air Soil Poll. 39, 409-20.
Cerkauskas, R.F., Dhingra, O.D., Sinclair, J.B. and Foor, S.R. (1982). Effect of three desiccant herbicides on soybean (Glycine max) seed quality. Weed Sci. 30, 484-90.
Clay, P.A. and Griffin, J.L. (2000). Weed seed production and seedling emergence responses to late-season glyphosate applications. Weed Sci. 48, 481-6.
Crawley, M.J., Hails, R.S., Rees, M., Kohn, D. and Buxton, J. (1993). Ecology of transgenic oilseed rape in natural habitats. Nature 363, 620-3.
Creager, R.A. (1992). Seed germination, physical and chemical control of catclaw mimosa (Mimosa pigra var. pigra). Weed Technol. 6(4), 884-91.
Darwent, A.L., Kirkland, K.J., Townley-Smith, L., Harker, K.N., Cessna, A.J., Lukow, O.M. and Lefkovitch, L.P. (1994). Effect of preharvest applications of glyphosate on the drying, yield and quality of wheat. Can. J. Plant Sci. 74, 221-30.
Egley, G.H. and Williams, R.D. (1978). Glyphosate and paraquat effects on weed seed germination and seedling emergence. Weed Sci. 26(3), 249-51.
Fernald, M.L. (1970). Gray's Manual of Botany, 8th edn. New York: D. van Nostrand Company.
Firbank, L.G. and Forcella, F. (2000). Genetically modified crops and farmland biodiversity. Science 289, 1481-2.
Freedman, B. (1991). Controversy over the use of herbicides in forestry, with particular reference to glyphosate usage. J. Environ. Sci. Health C8(2), 277-86.
Freemark, K.E. and Boutin, C. (1994). Impacts of Agricultural Use of Herbicides on Terrestrial Wildlife: A Review with Special Reference to Canada. Technical Report Series No. 196, Canadian Wildlife Service, Headquarters.
Ghassemi, M., Quinlivan, S. and Dellarco, M. (1982). Environmental effects of new herbicides for vegetation control in forestry. Environ. Int. 7, 389-401.
Gigax, D.R. and Burnside, O.C. (1976). Chemical desiccation of grain sorghum. Agronomy J. 68, 645-9.
Gleason, H.A. and Cronquist, A. (1991). Manual of Vascular Plants of Northeastern United States and Adjacent Canada. New York: New York Botanical Garden.
Grossbard, E. and Atkinson, D. (1985). The Herbicide Glyphosate. UK: Butterworths and Co. (Publishers) Ltd.
Gubbels, G.H., Bonner, D.M. and Kenaschuk, E.O. (1993). Effect of time of swathing and desiccation on plant drying, seed color and germination of flax. Can. J. Plant Sci. 73(4), 1001-7.
Hails, R.S. (2000). Genetically modified plants-the debate continues. Tree 15(1), 14-18.
Hassan, E.A. (1988). The influence of glyphosate on germination behaviour of wheat (Triticum aestivum L.). J. Agronomy Crop Sci. 161, 73-8.
Heap, I. (2002). The international survey of herbicide resistant weeds. Online. Internet. Available: www.weedscience.com.
Hurst, A. and John, E. (1999). The effectiveness of glyphosate for controlling Brachypodium pinnatum in chalk grassland. Biol. Conserv. 89, 261-5.
Isaacs, M.A., Murdock, E.C., Toler, J.E. and Wallace, S.U. (1989). Effects of late-season herbicide applications on sicklepod (Cassia obtusifolia) seed production and viability. Weed Sci. 37, 761-5.
Jepson, I., Martinez, A. and Sweetman, J.P. (1998). Chemical-Inducible gene expression systems for plants—a review. Pestic. Sci. 54, 360-7.
Kennedy, E.R. and Jordan, P.A. (1985). Glyphosate and 2,4-D: the impact of two herbicides on Moose browse in forest plantations. Alces 21, 1-11.
Kudoyarova, G.R., Teplova, I.R. and Cheredova, E.P. (1992). Effect of Glyphosate on content of indoleacetic and abscisic acids during germination of corn seeds. Soviet Plant Physiol. 39(3), 372-4.
Liu, L., Punja, Z.K. and Rahe, J.E. (1997). Altered root exudation and suppression of induced lignification as mechanisms of predisposition by glyphosate of bean roots (Phaseolus vulgaris L.) to colonization by Pythium spp. Physiol. Mol. Plant Pathol. 51(2), 110-27.
Monsanto Canada, Inc. (1991). Roundup Liquid Herbicide Agricultural and Industrial: Complete Directions for Use, Information Booklet.
Monsato Canada, Inc. (1992). Roundup Preharvest User's Guide.
Monsanto, (2001). Roundup Transorb®. http://www.agr.gouv.qc.ca/dgpar/rap/html/rech_pdf_arla.html.
Paoletti, M.G. and Pimentel, D. (1996). Genetic engineering in agriculture and the environment: assessing risks and benefits. BioScience 49(9), 665-73.
Payne, N., Feng, J. and Reynolds, P. (1987). Off-target Deposit Measurements and Buffers Required Around Water for Aerial Glyphosate Application. File Report No. 80. Forest Pest Management Institute: Canadian Forestry Service.
Pratley, J., Urwin, N., Stanton, R., Baines, P., Broster, J., Cullis, K., Schafer, D., Bohn, J. and Krueger, R. (1999). Resistance to glyphosate in Lolium rigidum. I. Bioevaluation. Weed Sci. 47, 405-11.
Ratnayake, S. and Shaw, D.R. (1992). Effects of harvest-aid herbicides on soybean (Glycine max) seed yield and Quality. Weed Technol. 6, 339-44.
Ribo, J.M. (1986). “Roundup” (Glyphosate): A TRC Technical Review.ToxicologyResearchCentre,UniversityofSaskatchewan.
Saroha, M.K., Sridhar, P. and Malik, V.S. (1998). Glyphosate-tolerant crops: Genes and enzymes. J. Plant Biochem. Biotechnol. 7, 65-72.
SAS Institute, (1999–2000). SAS/STAT User's Guide, Release 8.0. North Carolina, USA: SAS Institute, Cary.
Shaner, D.L. (2000). The impact of glyphosate-tolerant crops on the use of other herbicides and on resistance management. Pest Manag. Sci. 56, 320-6.
Shuma, J.M., Quick, W.A., Raju, M.V.S. and Hsiao, A.I. (1995). Germination of seeds from plants of Avena fatua L. treated with glyphosate. Weed Res. 35, 249-55.
Snow, A.A. and Palma, P.M. (1997). Commercialization of transgenic plants: potential ecological risks: will evolutionary effects of engineered crops exacerbate weed and pest problems? BioScience 47(2), 86-96.
Statistics Canada., (2001). Census of Agriculture. www. statcan.ca/english/censusag/can.htm.
Sutton, R.F. (1978). Glyphosate herbicide: an assessment of forestry potential. For. Chronicle 54(1), 24-8.
Trotter, D.M., Wong, M.P. and Kent, R.A. (1990). Canadian Water Quality Guidelines for Glyphosate. Environment Canada Scientific Series No. 170.
U.S. Department of Agriculture (USDA) (2002). National Biological Impact Program (http://www.nbiap.vt.edu). Biotechnology Industry Organization (http://www.bio.org/er/agri_products.asp).
Whigham, D.K., Stoller, E.W. (1979). Soybean desiccation by Paraquat, Glyphosate, and Ametryn to accelerate harvest. Agronomy J. 71, 630-3.
Woodburn, A.T. (2000). Glyphosate: production, pricing and use worldwide. Pest Manag. Sci. 56, 309-12.
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Blackburn, L.G., Boutin, C. Subtle Effects of Herbicide Use in the Context of Genetically Modified Crops: A Case Study with Glyphosate (Roundup®). Ecotoxicology 12, 271–285 (2003). https://doi.org/10.1023/A:1022515129526
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DOI: https://doi.org/10.1023/A:1022515129526