Abstract
Whole tissue extracts prepared from mouse brain regions at various postnatal ages were characterized for binding of factors to the DNase I hypersensitive site (HSS1) which is located closest to the transcription start site of the 68-kDa mouse neurofilament gene (NF-L). Gel mobility shift assays detected changes in factor binding during postnatal development of the neocortex. Competition experiments suggested that one of the complexes resulted from factor binding to a 9 bp sequence found in both the light and medium neurofilament promoter regions (NF-L/M). Gel mobility shifts performed with an oligonucleotide probe containing the NF-L/M sequence detected two brain-specific DNA-protein complexes, and a third complex in both brain and liver. During cerebellar and neocortical development, one of the NF-L/M complexes was most intense at postnatal day 10 when transcription of the NF-L gene is upregulated.
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Kure, R., Brown, I.R. Developmental Analysis of Factors Binding to the Mouse 68-kDa Neurofilament Promoter. Neurochem Res 22, 555–562 (1997). https://doi.org/10.1023/A:1022461817786
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DOI: https://doi.org/10.1023/A:1022461817786