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Influence of medium composition on callus induction and camptothecin(s) accumulation in Nothapodytes foetida

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Abstract

Camptothecin(s) production was examined in callus cultures derived from cotyledons of Nothapodytes foetida (Weigh) Sleumer. The calluses were grown on various combinations of Murashige and Skoog's basal media supplemented with auxins 2,4-dichlorophenoxyacetic acid (2,4-D), 2,4,5-trichlorophenoxyacetic acid (2,4,5-T), a-napthalene acetic acid (NAA) and indole-3-acetic acid (IAA) with 6-benzyl aminopurine (BA)/kinetin in different concentrations. The presence of camptothecin (CPT) and 9-methoxycamptothecin (9-OMeCPT) were analyzed by HPLC in relation to the media composition. Hyper production of CPT(1.306% on dry wt. basis) was observed with a combination of 2,4-D with BA and 2,4,5-T and NAA in 1-month-old callus.

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Authors and Affiliations

  1. Plant Tissue Culture Division, National Chemical Laboratory, Pune, 411008, India

    S.R. Thengane, D.K. Kulkarni, V.A. Shrikhande & K.V. Krishnamurthy

  2. Organic Chemistry Technology Division, National Chemical Laboratory, Pune, India

    S.P. Joshi & K.B. Sonawane

Authors
  1. S.R. Thengane
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  2. D.K. Kulkarni
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  3. V.A. Shrikhande
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  4. S.P. Joshi
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  5. K.B. Sonawane
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  6. K.V. Krishnamurthy
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Correspondence to S.R. Thengane.

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Thengane, S., Kulkarni, D., Shrikhande, V. et al. Influence of medium composition on callus induction and camptothecin(s) accumulation in Nothapodytes foetida . Plant Cell, Tissue and Organ Culture 72, 247–251 (2003). https://doi.org/10.1023/A:1022392929406

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  • DOI: https://doi.org/10.1023/A:1022392929406

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