Abstract
Japanese honeysuckle plant (Lonicera japonica Thunb.) is rich in iridoid secologanin and is a potentially useful model for the study of secologanin biosynthesis. Culture conditions for high frequency plant regeneration via somatic embryogenesis from zygotic embryo cultures and zygotic embryo-derived embryogenic cell suspension cultures of this species are described. Mature zygotic embryos formed embryogenic calluses at a frequency of 46.7% when cultured on Murashige and Skoog (MS) medium supplemented with 4.52 μM 2,4-dichloro-phenoxyacetic acid (2,4-D). Cell suspension cultures were established with embryogenic calluses using liquid MS medium with 4.52 μM 2,4-D. Upon plating onto MS basal medium, embryogenic cell suspension cultures produced numerous somatic embryos, which subsequently developed into plantlets at a frequency of 68%. Regenerated plantlets were transplanted to potting soil and grown to maturity in a greenhouse.
Similar content being viewed by others
References
Cambecedes J, Duron M & Decourtye L (1991) Adventitious bud regeneration from leaf explants of the shrubby ornamental hon-eysuckle, Lonicera nitida Wils. cv. Maigrun: effects of thidiazuron and 2,3,5-triodobenzoic acid. Plant Cell Rep. 10: 471-474
Chang WC & Hsu FL (1992) Inhibition of platelet activation and endothelial cell injury by polyphenolic compounds isolated from Lonicera japonica Thunb. Prost. Leuk. Essen. Fatty Acids 45: 307-312
Georges D, Chenieux J & Ochatt S (1993) Plant regeneration from aged-callus of the woody ornamental species Lonicera japonica cv. ‘Hall’s prolific’. Plant Cell Rep. 13: 91-94
Kawai H, Kuroyanagi M & Ueno A (1988) Iridoid glucosides from Lonicera japonica Thunb. Chem. Pharm. Bull. 36: 3664-3666
Lee SJ, Son KH, Chang HW, Kang SS & Kim HP (1998) Antiinflammatory activity of Lonicera japonica. Phytochem. Res. 12: 445-447
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco culture. Physiol. Plant. 15: 473-497
Palacios N, Christou P & Leech MJ (2002) Regeneration of Lonicera tatarica plants via adventitious organogenesis from cultured stem explants. Plant Cell Rep. 20: 808-813
Son KH, Jung KY, Chang HW, Kim HP & Kang SS (1994) Triterpenoid saponins from the aerial parts of Lonicera japonica. Phytochemistry 35: 1005-1008
Yamamoto H, Katano N, Ooi A, Inoue K (2000) Secologanin synthase which catalyzes the oxidative cleavage of loganin into secologanin is a cytochrome P450. Phytochemistry 53: 7-12
Zenk MH (1980) Enzymatic synthesis of ajmalicine and related indole alkaloids. J. Nat. Prod. 43: 438-451
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Kim, S.W., Cheol Oh, S., In, D.S. et al. High frequency somatic embryogenesis and plant regeneration in zygotic embryo cultures of Japanese honeysuckle. Plant Cell, Tissue and Organ Culture 72, 277–280 (2003). https://doi.org/10.1023/A:1022360115275
Issue Date:
DOI: https://doi.org/10.1023/A:1022360115275