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DNA fingerprinting and virulence analysis of Xanthomonas oryzae pv. oryzae isolates from Punjab, northern India

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Abstract

DNAs of 693 isolates of bacterial blight pathogen of rice, Xanthomonas oryzae pv. oryzae (Xoo), were characterized using PCR-based primers pJEL1 and pJEL2. The pathogen populations were grouped into 97 haplotypes based on DNA-banding patterns. An un-weighted pair-group method using arithmetic averages (UPGMA) indicated a high level of diversity in the pathogen isolates (51 lineages of Xoo at a 70% similarity level). Among these, lineages 5, 7, 27, and 29 are widely distributed and others are localized in the northern region of India. The isolates represent lineage-27, were prevalent in the entire disease-prone area in the region except at Ferozepur. Pathotyping data of the representative isolates of each lineage also indicate 17 different reaction patterns on a set of isogenic lines. Resistance genes xa8 and Xa21 were the most effective followed by xa5, and Xa7 against Xoo isolates prevalent in northern India. Different genes in combinations (xa5+xa13, xa5+Xa21, xa13+Xa21, and xa5+xa13+Xa21) in IR24 genetic background provided better protection against all the pathogen isolates tested in this study than did the component genes.

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Authors and Affiliations

  1. Biotechnology Center, Punjab Agricultural University, Ludhiana-, 141 004, India

    Sukhwinder-Singh, M. Sodhi, Y. Vikal, G. S. Bala, G. S. Mangat, M. Garg, J. S. Sidhu & H. S. Dhaliwal

  2. International Rice Research Institute, Manila, Philippines

    M. L. C. George

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  1. Sukhwinder-Singh
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  2. M. Sodhi
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  3. Y. Vikal
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  4. M. L. C. George
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  5. G. S. Bala
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  6. G. S. Mangat
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  7. M. Garg
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  8. J. S. Sidhu
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  9. H. S. Dhaliwal
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Sukhwinder-Singh, Sodhi, M., Vikal, Y. et al. DNA fingerprinting and virulence analysis of Xanthomonas oryzae pv. oryzae isolates from Punjab, northern India. Euphytica 130, 107–115 (2003). https://doi.org/10.1023/A:1022329024651

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