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Genotypic fidelity of micropropagated pineapple (Ananas comosus) plantlets assessed by isozyme and RAPD markers

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Abstract

In the present work, pineapple plantlets (cv. `Amarelinho') micropropagated by stationary (S) and temporary immersion (T) systems were evaluated in terms of genotypic fidelity by isozyme and RAPD markers. Neither isozymes (average 0.67%) nor RAPDs (average 7.5%) alone detected significant differences between the two micropropagated systems. However, when combined isozymes and RAPDs data more somaclonal variants were detected in S than T, with RAPDs revealing more variation than isozymes. In the T system, the treatment with PBZ 6.0 μM gave the greatest occurrence of variants (8.9%), although significant statistical differences (χ; p>0.05) between presence and absence of PBZ or GA were not detected. Temporary immersion in comparison with the stationary system resulted in the lowest proportion of somaclonal variants (1.9% vs. 3.9%). Although it does not represent an in depth genome analysis, ours is the first work that has attempted to evaluate the genotypic fidelity of micropropagated pineapple plantlets.

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Authors and Affiliations

  1. Laboratório de Fisiologia do Desenvolvimento e Genética Vegetal, Dep. de Fitotecnia, Universidade Federal de Santa Catarina, C.P. 476, Florianópolis SC, 88040-900, Brazil

    Sérgio Feuser, Kelen Meler, Miguel P. Guerra & Rubens O. Nodari

  2. Centro de Bioplantas, Universidad de Ciego de Ávila, CP 69450, Ciego de Ávila, Cuba

    Marcos Daquinta

Authors
  1. Sérgio Feuser
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  2. Kelen Meler
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  3. Marcos Daquinta
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  4. Miguel P. Guerra
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  5. Rubens O. Nodari
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Correspondence to Rubens O. Nodari.

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Feuser, S., Meler, K., Daquinta, M. et al. Genotypic fidelity of micropropagated pineapple (Ananas comosus) plantlets assessed by isozyme and RAPD markers. Plant Cell, Tissue and Organ Culture 72, 221–227 (2003). https://doi.org/10.1023/A:1022321405683

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