Abstract
Multiple shoot regeneration from the cut plumular ends of embryo axes of chickpea (Cicer arietinum L.) was evaluated on Murashige and Skoog medium having different concentrations of thidiazuron (TDZ) (0.1 to 10.0 mg dm−3) 6-benzylaminopurine (BAP) (0.5 and 1.0 mg dm−3), kinetin (0.5 and 1.0 mg dm−3) or zeatin (2.0 and 4.0 mg dm−3). TDZ (0.2 mg dm−3) was found to be the most effective cytokinin as it produced multiple shoots in 100 % of the explants from genotypes C235, ICC5166, ICC12269, ICC4951, ICC11531, BG256 and a local cultivar. Shoots were elongated on growth regulator-free medium, and rooted on growth regulator-free medium containing 1/4 MS salts + full vitamins + 3 % sucrose. Plantlets formed were acclimatized for 12 – 15 d in MS medium with a gradual reduction in sucrose concentration and transferred into pots filled with soil and kept in the field; this resulted in more than 70 % survival. The plants developed normally and produced fertile flowers and set seeds. Low temperatures, maximum 19.0 °C, and minimum 8.2 °C, during the first 15 d of transfer favoured survival on transfer to pots.
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Arnon, D.I.: Copper enzymes in isolated chloroplasts polyphenoloxidases in Beta vulgaris.-Plant Physiol 24: 1–15, 1949.
Barna, K.S., Wakhlu, A.K.: Somatic embryogenesis and plant regeneration from callus cultures of chickpea (Cicer arietinum L.).-Plant Cell Rep. 12: 521–524, 1993.
Boggetti, B., Jasik, J., Mantell, S.H.: In vitro root formation in Anacardium occidentale microshoots.-Biol. Plant. 44: 175–179, 2001.
Brandt, E.B., Hess, D.: In vitro regeneration and propagation of chickpea (Cicer arietinum L.) from meristem tips and cotyledonary nodes.-In Vitro cell. dev. Biol. Plant 30P: 75–80, 1994.
Eapen, S., George, L.: Somatic embryogenesis in Cicer arietinum L.: Influence of genotype and auxins.-Biol. Plant. 36: 343–349, 1994.
Fonnesbech, A., Fonnesbech, M.: Influence of cytokinins and temperature on development of Asparagus plumosus shoot tips in vitro.-Physiol. Plant. 45: 73–76, 1979.
Fontana, G.S., Santini, L., Caretto, S., Frugis, G., Mariotti, D.: Genetic transformation in the grain legume Cicer arietinum L. (chickpea).-Plant Cell Rep. 12: 194–198, 1993.
Gomez, K.A., Gomez, A.A.: Statistical Procedures for Agricultural Research.-John Wiley & Sons, New York 1984.
Hassanein, A.M., Soltan, D.M.: Solanum nigrum is a model system in plant tissue and protoplast cultures.-Biol. Plant. 43: 501–509, 2000.
Hita, O., Lafarga, C., Guerra, H.: Somatic embryogenesis from chickpea (Cicer arietinum L.) immature cotyledons: the effect of zeatin, gibberellic acid and indole-3-butyric acid.-Acta Physiol. Plant. 19: 333–338, 1997.
Hutchinson, M.J., Murch, S.J., Saxena, P.K.: Morphoregulatory role of thidiazuron: Evidence of the involvement of endogenous auxin in thidiazuron-induced somatic embryogenesis of geranium (Pelargonium × hortorum Bailey).-J. Plant Physiol. 149: 573–579, 1996.
Hyndman, S.E., Hasegawa, P.M., Bressan, R.A.: The role of sucrose and nitrogen in adventitious root formation on cultured rose shoots.-Plant Cell Tissue Organ Cult. 4: 229–238, 1982.
Kanyand, M., Dessai, A.P., Prakash, C.S.: Thidiazuron promotes high frequency regeneration of peanut (Arachis hypogaea) plants in vitro.-Plant Cell Rep. 14: 1–5, 1994.
Kar, S., Johnson, T.M., Nayak, P., Sen, S.K.: Efficient transgenic plant regeneration through Agrobacterium-mediated transformation of chickpea (Cicer arietinum L.).-Plant Cell Rep. 16: 32–37 1996.
Koroch, A.R., Juliani, H.R., Jr., Juliani, H.R., Trippi, V.S.: Micropropagation and acclimatization of Hedeoma multiflorum.-Plant Cell Tissue Organ Cult. 48: 213–217, 1997.
Kumar, V.D., Kirti, P.B., Sachan, J.K.S., Chopra, V.L.: Plant regeneration via somatic embryogenesis in chickpea (Cicer arietinum L.).-Plant Cell Rep. 13: 468–472, 1994.
Langford, P.J., Wainright, H.: Effect of sucrose concentration on the photosynthetic ability of rose shoots in vitro.-Ann. Bot. 60: 633–640, 1987.
Lu, C.: The use of thidiazuron in tissue culture.-In Vitro cell. dev. Biol. Plant 29P: 92–96, 1993.
Malik, K.A., Saxena, P.K.: Thidiazuron induces high-frequency regeneration in intact seedlings of pea (Pisum sativum), chickpea (Cicer arietinum) and lentil (Lens culinaris).-Aust. J. Plant Physiol. 19: 731–740, 1992.
Murashige, T., Skoog, F.A.: Revised medium for rapid growth and bioassays with tobacco tissue cultures.-Physiol. Plant. 15: 473–497, 1962.
Polisetty, R., Patil, P., Deveshwar, J.J., Khetarpal, S., Suresh, K., Chandra, R.: Rooting and establishment of in vitro grown shoot tip explants of chickpea (Cicer arietinum L.).-Indian J. exp. Biol. 34: 806–809, 1996.
Polisetty, R., Paul, V., Deveshwar, J.J., Khetarpal, S., Suresh, K., Chandra, R.: Multiple shoot induction by benzyladenine and complete plant regeneration from seed explants of chickpea (Cicer arietinum L.).-Plant Cell Rep. 16: 565–571, 1997.
Radhakrishnan, T., Murthy, T.G.K., Chandran, K., Bandyopadhyay, A.: Micropropagation in peanut (Arachis hypogaea L.).-Biol. Plant. 43: 447–450, 2000.
Serret, M.D., Trillas, M.I., Matas, J., Araus, J.L.: Development of photoautotrophy and photoinhibition of Gardenia jasminoides plantlets during micropropagation.-Plant Cell Tissue Organ Cult. 45: 1–6, 1996.
Shri, P.V., Davis, T.M.: Zeatin induced shoot regeneration from immature chickpea (Cicer arietinum L.) cotyledons.-Plant Cell Tissue Organ Cult. 28: 45–51, 1992.
Srivastava, K., Tiwari, K.N., Singh, R., Singh, B.D., Jaiswal, H.K.: Shoot regeneration from immature cotyledons of Cicer arietinum.-Biol. Plant. 44: 333–337, 2001.
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Singh, R., Srivastava, K., Jaiswal, H. et al. High Frequency Multiple Shoot Regeneration from Decapitated Embryo Axes of Chickpea and Establishment of Plantlets in the Open Environment. Biologia Plantarum 45, 503–508 (2002). https://doi.org/10.1023/A:1022308605583
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DOI: https://doi.org/10.1023/A:1022308605583