Journal of Chemical Ecology

, Volume 29, Issue 1, pp 253–257 | Cite as

Odor Compound Detection in Male Euglossine Bees

Article

Abstract

Male euglossine bees collect fragrances from various sources, which they store and use for as yet unknown purposes. They are attracted, often specifically, to single odor compounds and blends thereof. We used gas chromatography with electroantennographic detection (GC-EAD) and electroantennography (EAG) to investigate the response to 8 odor compounds by males of two euglossine species, Euglossa cybelia Moure and Eulaema polychroma (Mocsàry). In E. cybelia, we recorded EAD reactions in response to 1,8-cineole, methyl benzoate, benzyl actetate, methyl salicylate, eugenbl, and methyl cinnamate. E. polychroma responded to the same compounds in EAG experiments, while (ls)(-)α-pinene and β-pinene failed to trigger EAD or EAG responses in the bees. Blends of two compounds triggered larger responses than single compounds in EAG experiments with E. polychroma, however, when α-pinene was added, reactions decreased. In the light of existing data on the bees' behavior towards these odor compounds, our work indicates that both peripheral and central nervous processes influence the attraction of euglossine bees to odors.

Euglossine bees GC-EAD EAG odor compound detection 

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References

  1. Ackerman, J. D. 1989. Geographic and seasonal variation in fragrance choices and preferences of male Euglossine bees. Biotropica 21:340-347.Google Scholar
  2. Cromarty, S. I. and Derby, C. D. 1998. Inhibitory receptor binding events among the components of complex mixtures contribute to mixture suppression in responce of olfactory receptor neurons of spiny lobsters. J. Camp. Physiol. A 183:699-707.Google Scholar
  3. Dodson, C. H., Dresslbr, R. L., Hills, H. G., Adams, R. M., and Williams, N. H. 1969. Biologically active compounds in orchid fragrances. Science 164:1243-1249.Google Scholar
  4. Eltz, T., Whitten, W. M., Roubik, D. W., and Linsenmair, K. E. 1999. Frangrance collection, storage, and accumulation by individual male orchid bees. J. Chem. Ecol. 25:157-176.Google Scholar
  5. Hills, H. G., Williams, N. H., and Dodson, C. H. 1972. Floral fragrances and isolating mechanisms in the genus Catasetum (Orchidaceae). Biotropica 4:61-76.Google Scholar
  6. Roubik, D. W. and Hanson, P. E. (in press). Orchid bees: Biology and field guide. In Bio Press. Heredia, Costa Rica.Google Scholar
  7. Smith, B. H. and Getz, W. M. 1994. Nonpheromonal olfactory processing in insects. Anna. Rev. Entomol 39:351-375.Google Scholar
  8. Vogel, S. 1966. Parfümsammelnde Bienen als Bestäuber von Orchidaceen und Gloxinia. österr. Bot. Zeitschr. 113:302-361.Google Scholar
  9. Williams, N. H. and Whitten, W. M. 1983. Orchid floral fragrances and male euglossine bees: methods and advances in the last sesquidecade. Biol. Bull. 164:355-395.Google Scholar
  10. Williams, N. H. and Dodson, C. H. 1972. Selective attraction of male euglossine bees to orchid floral fragrances and its importance in long distance pollen flow. Evolutio. 26:84-95.Google Scholar

Copyright information

© Plenum Publishing Corporation 2003

Authors and Affiliations

  1. 1.Geobotanical Institute ETHZürich
  2. 2.Smithsonian Tropical Research Institute, ApartadoBalboaRepublic of Panama

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