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Setup for Stimulating and Measuring the Electrical Activity of an Olfactory Epithelium

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Abstract

A setup for recording the electrical activity of an insulated olfactory epithelium is described. The setup consists of the following basic elements: an electrophysiological chamber intended for supporting the vital activity of the olfactory epithelium, its isolation from outside smells, and stimulation by odor stimuli; a stimulation system intended for preparation of a saturated vapor of the stimulating substance, its dilution down to concentrations of 10–4–5.6 × 10–2 and application of air stimuli with a duration of 1–5 s and repetition period of 2–100 s; and a recording and control system, which is controlled manually or via a computer and synchronizes the stimulus application and olfactogram recording (K amp = 10 000, sensitivity is 10 μV, bandwidth is 0–100 Hz) and automatic compensation of the transepithelial potential drift.

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Authors and Affiliations

  1. Institute of Cell Biophysics, Russian Academy of Sciences, ul. Institutskaya 3, Pushchino, Moscow oblast, 142292, Russia

    A. A. Khokhlov & S. S. Kolesnikov

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  1. A. A. Khokhlov
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  2. S. S. Kolesnikov
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Khokhlov, A.A., Kolesnikov, S.S. Setup for Stimulating and Measuring the Electrical Activity of an Olfactory Epithelium. Instruments and Experimental Techniques 45, 834–837 (2002). https://doi.org/10.1023/A:1021451726808

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