Abstract
The titre of apple chlorotic leaf spot virus (ACLSV) was higher in microplants of Malus domestica cv. Jonagold than in 2-year-old grafted scions. Cytokinin concentration in the medium increased the titre of prunus necrotic ringspot virus (PNRSV) in the apex of microplants of Prunus insititia cv. Kozlienka but did not affect the titre of ACLSV in M .domestica.Virus titre of ACLSVwas higher in the haulms of autotrophically-grown compared with heterotrophically-grown microplants whereas as for PNRSV the results were the reverse. For both viruses, however, titre of the virus in the roots of autotrophically-grown plants was significantly higher than in haulm tissue from heterotrophic cultures. Ribavirin incorporation resulted in elimination of both viruses. Negative ELISA results were confirmed independently by PCR. The efficacy of Ribavirin in elimination of ACLSV was increased by increasing the concentration of cytokinin in the medium in parallel with decreasing the concentration of Ribavirin. These results are discussed in the context of the reliability of in vitro virus testing.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Agrios GN (2001) Plant Pathology. Academic Press, London.
Bailiss KW, Cocker FM & Cassells AC (1977) The effect of benlate and cytokinins on the content of tobacco mosaic virus in tomato leaf disks and cucumber mosaic virus in cucumber cotyledon disks and seedlings. Ann. Appl. Biol. 87: 383-392
Barrett C & Cassells AC (1994) An evaluation of antibiotics for the elimination of Xanthomonas campestris pv. pelargonii (Brown) from PelargoniumΧdomesticum cv. Grand Slam explants in vitro. Plant Cell Tiss. Org. Cult. 36: 169-175
Cassells AC (1996) Factors relevant to the use of Ribavirin to eliminate viruses in vitro In: Physiology and control of plant propagation in vitro. Proceedings of COST 822 Workshop (pp 144-148). Humboldt University, Berlin
Cassells AC & Long RD (1983) The effect of 1-b-ribofuranosyl-1, 2, 4-triazole-3-carboxamide on bud and shoot formation in tobacco petiole cultures. Physiol. Plant. 59: 664-668
Cassells AC & O'Herlihy EA (2001) Pathogen elimination and contamination management. In: Jain SM (ed) Micropropagation of Woody Trees and Fruits. Kluwer, Dordrecht (in press)
Cassells AC, Doyle BM & Curry RF (eds.) (2000) Methods and markers for quality assurance in micropropagation. Acta Hortic. 530
EPPO (1998) Certification Schemes PM4/1-26 European and Mediterranean Plant Protection Organization, Paris
George EF (1993) Plant Propagation by Tissue Culture: Part 1 - The Technology Exegetics, Basingstoke
Kummert J, Vendrame M, Lepoivre P & Steyer S (2001) Development of routine RT-PCR ELOSA tests for fruit tree certification. Acta Hortic. 550: 45-52
Knapp E, Hanzer V, Weiss H, da Camara Machado A, Katinger H & Laimer da Camara Machado M (1996) Towards certification of plant stocks in vitro In: Physiology and control of plant propagation in vitro. Proceedings of COST 822Workshop (pp 137-143). Humboldt University, Berlin
Leonhardt W, Wawrosch C, Auer A & Kopp B (1997) Monitoring of virus disease in Austrian grapevine varieties and virus elimination using in vitro thermotherapy. In: Cassells AC (ed) Pathogen and Microbial Contamination Management in Micropropagation (pp 21-218). Kluwer, Dordrecht
Matthews REF (1991) Plant Virology. Academic Press, New York.
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant 15: 473-497
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
O'Herlihy, E., Croke, J. & Cassells, A. Influence of in vitro factors on titre and elimination of model fruit tree viruses. Plant Cell, Tissue and Organ Culture 72, 33–42 (2003). https://doi.org/10.1023/A:1021260202876
Issue Date:
DOI: https://doi.org/10.1023/A:1021260202876