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The Neurite-Stimulating Influence of Components of Medicinal Leech Salivary Gland Secretions in Organotypic Culture of Spinal Ganglia

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Abstract

The effects of components from medicinal leech (Hirudo medicinalis) salivary gland secretions and the therapeutic agent Piyavit on the growth of chick embryo neurites in organotypic culture were studied. Native destabilase and bdellin A at concentrations of 0.01, 0.02, 0.05, and 0.1 ng/ml, bdellin B at a concentration of 0.05 ng/ml, and eglin at a concentration of 0.1 ng/ml had neurite-stimulating activity, evident on the third day of organotypic culture of spinal ganglia. The stimulatory activity of destabilase was lost after revere-phase chromatography. The neurite-stimulating activity of the extract of the therapeutic agent Piyavit (200 ng/ml) in organotypic ganglion culture appeared to result from the neurite-stimulating salivary gland components within this agent, suggesting that Piyavit could be used for the treatment of neurodegenerative disorders.

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Authors and Affiliations

  1. Laboratory for Excitable Membrane Physiology, I. P. Pavlov Institute of Physiology, Russian Academy of Sciences, 6 Makarov Bank, 199034, St. Petersburg, Russia

    N. I. Chalisova & V. A. Pennijainen

  2. Faculty of Biology, M. V, Lomonosov Moscow State University, Vorob'ev Heights, 119899, Moscow, Russia

    I. P. Baskova

  3. Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117871, Moscow, Russia

    L. L. Zavalova

Authors
  1. N. I. Chalisova
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  2. I. P. Baskova
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  3. L. L. Zavalova
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  4. V. A. Pennijainen
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Chalisova, N.I., Baskova, I.P., Zavalova, L.L. et al. The Neurite-Stimulating Influence of Components of Medicinal Leech Salivary Gland Secretions in Organotypic Culture of Spinal Ganglia. Neurosci Behav Physiol 33, 85–88 (2003). https://doi.org/10.1023/A:1021187500632

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