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Agar Cultures of Human Clonogenic Hemopoietic Precursor Cells for Early Diagnosis of Some Myeloproliferative Diseases

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Abstract

Growth characteristics of human hemopoietic cells in erythremia and chronic myeloid leukemia were studied using agar cultures with and without hemopoietic growth factors. Agar cultures, similarly to cultures on other semisolid media (plasma clot, methylcellulose) can be used for early differential diagnosis of polycythemia vera (erythremia) and secondary erythrocytosis: erythremia, but not erythrocytosis, is characterized by spontaneous (erythropoietin-independent) formation of colonies from erythrocyte precursor cells. Spontaneous colony formation from granulocyte-macrophage precursor cells can serve as an important test for early diagnosis of chronic myeloid leukemia. The study of colony formation from granulocyte-macrophage precursors and of the capacity of bone marrow cells to form colonies from hemopoietic stromal precursor cells revealed new characteristics of the studied myeloproliferative diseases. Presumably, spontaneous colony formation from erythrocytic and myeloid precursors should be regarded as a sign of tumor transformation of the studied hemopoietic cells.

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Authors and Affiliations

  1. Medical Radiology Research Center, Russian Academy of Medical Sciences, Obninsk

    A. I. Kolesnikova, V. V. Pavlov, A. G. Konoplyannikov, L. A. Lepekhina, S. Sh. Kal'sina, M. A. Danilova, E. V. Domracheva & A. I. Vorob'ev

  2. Hematological Research Center, Russian Academy of Medical Sciences, Moscow

    A. I. Kolesnikova, V. V. Pavlov, A. G. Konoplyannikov, L. A. Lepekhina, S. Sh. Kal'sina, M. A. Danilova, E. V. Domracheva & A. I. Vorob'ev

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  1. A. I. Kolesnikova
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  2. V. V. Pavlov
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  3. A. G. Konoplyannikov
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  4. L. A. Lepekhina
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  5. S. Sh. Kal'sina
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  6. M. A. Danilova
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  7. E. V. Domracheva
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  8. A. I. Vorob'ev
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Kolesnikova, A.I., Pavlov, V.V., Konoplyannikov, A.G. et al. Agar Cultures of Human Clonogenic Hemopoietic Precursor Cells for Early Diagnosis of Some Myeloproliferative Diseases. Bulletin of Experimental Biology and Medicine 134, 181–186 (2002). https://doi.org/10.1023/A:1021152702332

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  • DOI: https://doi.org/10.1023/A:1021152702332

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