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Regeneration of Small Intestinal Mucosa After Acute Ischemia–Reperfusion Injury

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Abstract

To determine whether c-Fos and c-Jun are involved in the repair of small intestinal mucosa after ischemia–reperfusion (I/R), we investigated the mechanism of regeneration following acute I/R injury in rats by evaluating changes in DNA synthesis, fractional synthesis rate (FSR) of proteins, and alkaline phosphatase (ALP) activity. Furthermore, we examined the sequential expression of c-Fos and c-Jun using western blot analysis and immunohistochemical staining. Proliferating cell nuclear antigen (PCNA) labeling index (LI) demonstrated that the LI of the I/R group at 2 and 6 hr after reperfusion was significantly higher than that of the controls. Statistically significant differences were found between the FSRs of the I/R group and the corresponding conventional group at 2, 6, and 12 hr. The expression of c-Fos and c-Jun proteins increased markedly after I/R and these proteins decreased with time. The levels of ALP in the I/R group were significantly decreased at 2 and 6 hr after reperfusion compared to controls. These results indicate that c-Fos and c-Jun play a central role in the repair process that results in complete restoration of intestinal mucosal function after I/R.

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Authors and Affiliations

  1. Department of Surgery (II), School of Medicine, Kanazawa University, Ishikawa, 920-8641, Japan

    Hiroshi Itoh, Masao Yagi, Ken Hasebe, Sachio Fushida, Takashi Tani, Tetsuo Hashimoto, Kouichi Shimizu & Koichi Miwa

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  1. Hiroshi Itoh
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  2. Masao Yagi
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  3. Ken Hasebe
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  4. Sachio Fushida
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  5. Takashi Tani
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  6. Tetsuo Hashimoto
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  7. Kouichi Shimizu
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  8. Koichi Miwa
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Itoh, H., Yagi, M., Hasebe, K. et al. Regeneration of Small Intestinal Mucosa After Acute Ischemia–Reperfusion Injury. Dig Dis Sci 47, 2704–2710 (2002). https://doi.org/10.1023/A:1021049004188

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