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Co-localisation of Nitric Oxide Synthase and Endothelin in the Rat Supraoptic Nucleus

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Abstract

The co-localisation of neuronal nitric oxide synthase and endothelin-1 was studied in the rat supraoptic nucleus at the electron microscopy level. Double pre-embedding immunocytochemistry was performed using ExtrAvidin-horseradish peroxidase and immunogold–silver techniques. Immunoreactivities to neuronal nitric oxide synthase and endothelin-1 were co-localised in sub-populations of endocrine neurones (cell bodies) and dendrites. Double-labelled axon terminals making asymmetrical synapses on unlabelled dendrites were also observed. The findings are discussed in terms of the possible role and significance of nitric oxide and endothlin-1 in the hypothalamo-neurohypophysial system.

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  1. Department of Anatomy and Developmental Biology and Centre for Neuroscience, University College London, Gower Street, London, WC1E 6BT, UK

    Andrew B. Mukherjee & Andrzej Loesch

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  1. Andrew B. Mukherjee
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  2. Andrzej Loesch
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Mukherjee, A.B., Loesch, A. Co-localisation of Nitric Oxide Synthase and Endothelin in the Rat Supraoptic Nucleus. Histochem J 34, 181–187 (2002). https://doi.org/10.1023/A:1020950700078

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