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Magnetic polysiloxane-polyvinyl alcohol composite as solid-phase in chemiluminescent assays

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Abstract

A polysiloxane and polyvinyl alcohol interpenetrating polymer network was synthesised and its ferromagnetic derivative was used as solid support for antigen covalent immobilisation in chemiluminescent assays. Only 0.625 μg of either Trypanosoma cruzi or Schistosoma mansoni antigens immobilized onto the magnetic particles (2.5 mg) were sufficient to detect the correspondent human IgG within a nanogram scale.

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Authors and Affiliations

  1. Laboratório de Imunopatologia Keizo Asami and Departamento de Bioquímica, Universidade Federal de Pernambuco, Cidade Universítaria, 50670-420, Recife, Brazil

    R.A.L. Coêlho, G.A. Jaques, A.D. Barbosa & L.B. Carvalho Jr.

  2. Instituto de Investigaciones en Ciencias de la Salud, Universidad Nacional de Asuncion, Asuncion, Paraguay

    G. Velazquez

  3. Centro de Pesquisas Aggeu Magalhães, FIOCRUZ, Pernambuco, Brazil

    S.M.L. Montenegro

  4. Departamento de Química Fundamental, Universidade Federal de Pernambuco, Cidade Universítaria, 50670-420, Recife, Brazil

    W.M. Azevedo

Authors
  1. R.A.L. Coêlho
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  2. G.A. Jaques
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  3. A.D. Barbosa
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  4. G. Velazquez
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  5. S.M.L. Montenegro
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  6. W.M. Azevedo
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  7. L.B. Carvalho Jr.
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Coêlho, R., Jaques, G., Barbosa, A. et al. Magnetic polysiloxane-polyvinyl alcohol composite as solid-phase in chemiluminescent assays. Biotechnology Letters 24, 1705–1708 (2002). https://doi.org/10.1023/A:1020675815637

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  • DOI: https://doi.org/10.1023/A:1020675815637

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