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Rapid detection of a minute amount of lipopolysaccharide from Salmonella in large volumes by poly(ethyleneimine)-cloth enzyme immunoassay

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Abstract

Utilizing the rapidity in capturing Salmonella lipopolysaccharide (LPS) and the macroporous nature of poly(ethyleneimine) immobilized polyester cloth (PEI-cloth), a PEI-cloth disk set in a Swinnex cartridge joined at both ends with 10 ml plastic syringes was repeatedly passed through with an LPS sample in large volumes. The captured LPS on the disk were detected at up to 1 pg, which is equivalent to the amount produced of 16 cells, by cloth enzyme immunoassay.

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References

  • Blais B, Yamazaki H (1989) Rapid antibody assay based on initial rate of immuno reaction. Biotechnol. Tech. 3: 253-256.

    Google Scholar 

  • Blais B, Yamazaki H (1996) Method for using polymyxincoated substrate for lipopoysaccharide detection. US Patent No. 5,510,242.

  • Boyd S, Yamazaki H (1995) Detection of dilute antigen in large sample volumes using antibody-coated polyester cloth. Biotechnol. Tech. 9:849-852.

    Google Scholar 

  • Button L, Yamazaki H (1997) Rapid detection of dilute lipopolysaccharide antigens in large volumes using polymyxin-coated polyester cloth. Biotechnol. Tech. 11: 475-478.

    Google Scholar 

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Authors and Affiliations

  1. Department of Applied Chemistry & Biochemistry, Faculty of Engineering, Kumamoto University, 2-39-1 Kurokami, Kumamoto, 860-8555, Japan

    Kouchi Matsumoto, Masayo Sakata, Masashi Kunitake & Chuichi Hirayama

  2. Ricoh Kyosan, Inc., Yaesu-Mitsui Bldg, 2-7-2 Yaesu, Chuo-ku, Tokyo, 104-0028, Japan

    Masao Miyazakia

Authors
  1. Kouchi Matsumoto
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  2. Masayo Sakata
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  3. Masashi Kunitake
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  4. Masao Miyazakia
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  5. Chuichi Hirayama
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Matsumoto, K., Sakata, M., Kunitake, M. et al. Rapid detection of a minute amount of lipopolysaccharide from Salmonella in large volumes by poly(ethyleneimine)-cloth enzyme immunoassay. Biotechnology Letters 24, 1791–1795 (2002). https://doi.org/10.1023/A:1020669422965

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  • DOI: https://doi.org/10.1023/A:1020669422965

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