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A caprylate esterase–activated fluorescent probe for sensitive and selective detection of Salmonella enteritidis

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Abstract

Salmonella enteritidis is one of the most common foodborne pathogens. Many methods have been developed to detect Salmonella, but most of them are expensive, time-consuming, and complex in experimental procedures. Developing a rapid, specific, cost-effective, and sensitive detection method is still demanded. In this work, a practical detection method is presented using salicylaldazine caprylate as the fluorescent probe, which could be hydrolyzed by caprylate esterase liberated from Salmonella lysed by phage, to form strong fluorescent salicylaldazine. The Salmonella could be detected accurately with a low limit of detection of 6 CFU/mL and a broad concentration range of 10–106 CFU/mL. Moreover, this method was successfully used for the rapid detection of Salmonella in milk within 2 h through pre-enrichment by ampicillin-conjugated magnetic beads. The novel combination of fluorescent turn-on probe salicylaldazine caprylate and phage ensures this method has excellent sensitivity and selectivity.

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Funding

This work was financially supported by the Fundamental Research Funds for the Central Universities (No. 2662019QD032) and the National Key Research and Development Program of China (2017YFE0113900).

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Authors and Affiliations

  1. College of Science, Huazhong Agricultural University, Wuhan, 430070, China

    Haotian Zhang, Zuhui Xu, Jimei Ma, Zi-Long Li, Wan-Min Cheng & Hong Jiang

  2. College of Food Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China

    Xiaohong Wang

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  1. Haotian Zhang
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Zhang, H., Wang, X., Xu, Z. et al. A caprylate esterase–activated fluorescent probe for sensitive and selective detection of Salmonella enteritidis. Anal Bioanal Chem 415, 2163–2172 (2023). https://doi.org/10.1007/s00216-023-04623-9

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