Skip to main content
SpringerLink
Log in

A Natural Triploid in Trichomycterus Davisi (Siluriformes, Trichomycteridae): Mitotic and Meiotic Characterization by Chromosome Banding and Synaptonemal Complex Analyses

  • Published:
Genetica Aims and scope Submit manuscript

Abstract

Within a total of 50 analyzed specimens a male individual of Trichomycterus davisi has been recorded with 81 chromosomes including 60 metacentric, 18 submetacentric and three subtelocentric chromosomes. When compared with diploid individuals (2n = 54) and the morphological standard of chromosomes, this male is a triploid with 3n = 81 chromosomes. Since staining with silver nitrate indicates three active nucleolar organizer regions (NORs), the three NOR-bearing chromosomes in this individual are genetically active. Analysis of the synaptonemal complex (SC) by electronic microscopy shows that there is an incomplete pairing of the third set of chromosomes in the triploid individual.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Almeida-Toledo, L.F., F. Foresti & S.A. de Toledo Filho, 1985. Spontaneous triploidy and NOR activity in Eigenmannia sp. (Pisces, Sternopygidae) from the Amazon basin. Genetica 66: 85–88.

    Google Scholar 

  • Al-Sabti, K., B. Kurelec & N. Fijan, 1983. Spontaneous triploidy and tetraploidy in the common carp (Cyprinus carpio L.). Vet. Arhiv. 53(5): 217–223.

    Google Scholar 

  • Amemiya, C.T. & J.R. Gold, 1986. Chromomycin A3 stains nucleolus organizer regions of fish chromosomes. Copeia 1986: 226–231.

    Google Scholar 

  • Bertollo, L.A.C. & C.A. Mestriner, 1998. The X1X2Y sex chromosome system in the fish Hoplias malabaricus. II. Meiotic analyses. Chromosome Res. 6: 141–147.

    Google Scholar 

  • Bertollo, L.A.C., C.S. Takahashi & O. Moreira-Filho, 1978. Cytotaxonomic considerations on Hoplias lacedae (Pisces, Erythrinidae). Rev. Bras. Genet. 1: 103–120.

    Google Scholar 

  • Borin, L.A. & I.C. Martins-Santos, 1999. Karyotype characterization of three species of the genus Trichomycterus (Teleostei, Siluriformes, from Iguaçu river. Genetica 106: 215–221.

    Google Scholar 

  • Centofante, L., L.A.C. Bertollo & O. Moreira-Filho, 2001. Comparative cytogenetics among sympatric species of Characidium (Pisces, Characiformes). Diversity analysis with the description of a ZW sex chromosome system and natural triploidy. Caryologia 54(3): 253–260.

    Google Scholar 

  • Cuellar, O. & T. Uyeno, 1972. Triploidy in rainbow trout. Cytogenetics 11: 508–515.

    Google Scholar 

  • Dias, A.L., F. Foresti & C. Oliveira, 1998. Synapsis in supernumerary chromosomes of Prochilodus lineatus (Teleostei, Prochilodontidae). Caryologia 51(2): 105–113.

    Google Scholar 

  • Evans, G.M. & E.W. Davies, 1983. Fertility and stability of induced polyploids, 139 pp. in Kew Chromosome Conference II, edited by P.E. Brandham & M.D. Bennett. Allen and Unwin, London.

    Google Scholar 

  • Fauaz, G., V.E. Vicente & O. Moreira-Filho, 1994. Natural triploidy and B chromosomes in the neotropical fish genus Astyanax (Characidae). Brazil J. Genet. 17(2): 157–163.

    Google Scholar 

  • Foresti, F., C. Oliveira, P.M. Galetti Jr. & L.F. Almeida-Toledo, 1993. Synaptonemal complex analysis in spermatocytes of tilapia, Oreochromis niloticus (Pisces, Cichlidae). Genome 36: 1124–1128.

    Google Scholar 

  • Galetti Jr., P.M.& E.M. Rasch, 1993. NOR variability in diploid and triploid forms of the amazon molly Poecilia formosa as shown by silver nitrate and chromomycin A3 staining. Rev. Bras. Genet. 16(4): 927–938.

    Google Scholar 

  • Gilles, C.B., 1984. The synaptonemal complex in higher plants. CRC Crit. Rev. Plant Sci. 2(2): 81–116.

    Google Scholar 

  • Giuliano-Caetano, L. & L.A.C. Bertollo, 1990. Karyotypic variability in Hoplerythrinus unitaeniatus (Pisces, Characiformes, Erythrinidae). II. Occurrence of natural triploidy. Brazil J. Genet. 13(2): 231–237.

    Google Scholar 

  • Gold, J.R. & J.C. Avise, 1976. Spontaneous triploidy in the California roach Hesperoleucus symmetricus (Pisces, Cyprinidae) Cytogenet. Cell Genet. 17: 144–149.

    Google Scholar 

  • Howell, W.M., & D.A. Black, 1980. Controlled silver-staining of nucleolus organizer regions with a protective colloidal developer: a 1-step method. Experientia 36: 1014–1015.

    Google Scholar 

  • Loidl, J., K. Nairz & F. Klein, 1991. Meiotic chromosome synapsis in a haploid yeast. Chromosoma 100: 221–228.

    Google Scholar 

  • Lozano, R., C. Ruiz Rejón & M. Ruiz Rejón, 1992. A comparative analysis of NORs in diploid and triploid salmonids: implications with respect to the diploidization process occurring in this fish group. Heredity 69: 450–457.

    Google Scholar 

  • Malacrida, A.C.C.P., 2002. Características cromossômicas de algumas espécies de Astyanax (Characidae, Tetragonopterinae) da bacia do rio Tibagi, PR. Dissertação de Mestrado. Universidade Estadual de Londrina, Londrina, PR, Brasil, 95 pp.

    Google Scholar 

  • Mestriner, C.A., L.A.C. Bertollo & P.M. Galetti Jr., 1995. Chromosome banding and synaptonemal complex in Leporinus lacustris (Pisces, Anostomidae): analysis of a sex system. Chromosome Res. 3: 440–443.

    Google Scholar 

  • Mestriner, C.A., P.M. Galetti Jr., S.R. Valentini, I.R.G. Ruiz, L.D.S. Abel, O. Moreira-Filho & J.P.M. Camacho, 2000. Structural and functional evidence that a B chromosome in the characid fish Astyanax scabripinnis is an isochromosome. Heredity 85: 1–9.

    Google Scholar 

  • Morelli, S., A.C. Bertollo & O. Moreira-Filho, 1983. Citogenetics considerations on the genus Astyanax (Pisces, Characidae). II. Occurrence of the natural triploidy. Caryologia 36(3): 245–250.

    Google Scholar 

  • Ohno, S., 1970. Evolution By Gene Evolution. Springer Verlag, New York.

    Google Scholar 

  • Ohno, S., 1974. Animal Citogenetics. Vol. 4, Chordata 1, Gerbruder Borntraeger, Berlin.

    Google Scholar 

  • Oliveira, C., F. Foresti, M.G. Rigolino & Y.A. Tabata, 1995a. Synaptonemal complex analysis in spermatocytes and oocytes of rainbow trout, Oncorhynchus mykiss (Pisces, Salmonidae): the process of autosome and sex chromosome synapsis. Chromosome Res. 3: 182–190.

    Google Scholar 

  • Oliveira, C., F. Foresti, M.G. Rigolino & Y.A. Tabata, 1995b. Synaptonemal complex formation in spermatocytes of the autotriploid rainbow trout, Oncorhynchus mykiss (Pisces, Salmonidae). Hereditas 123: 215–220.

    Google Scholar 

  • Oliveira, C., F. Foresti, M.G. Rigolino & Y.A. Tabata, 1996. Paracentric inversion involving a NOR-bearing chromosome of rainbow trout (Oncorhynchus mykiss): electron microscopy studies of the synaptonemal complex. Caryologia 49(3-4): 335–342.

    Google Scholar 

  • Portela-Castro, A.L.B., H.F. JÚlio-Junior & P.B. Nishiyama, 2001. New occurrence of microchromosomes B in Moenkhausia sanctafilomenae (Pisces, Characidae) from the Paraná river of Brazil: analysis of the synaptonemal complex. Genetica 110: 277–283.

    Google Scholar 

  • Rasmussen, S.W., 1977. Chromosome pairing in triploid females of Bombyx mori analysed by three dimensional reconstruction of synaptonemal complexes. Carlsberg Res. Commun. 46: 347–360.

    Google Scholar 

  • Romashov, D.D. & V.N. Belyaeva, 1965. Apud Valenti, R.J., 1975. Induced polyploidy in Tilapia aurea (Steindachner) by means of temperature shock treatment. J. Fish Biol. 7: 519–528.

    Google Scholar 

  • Santos, J.L., 1993. Spreading synaptonemal complex from the grasshopper Clorthippus jacobsi: pachytene and zygotene observations. Hereditas 118: 235–241.

    Google Scholar 

  • Schmid, M. 1982. Chromosome banding in Amphibia. VII. Analysis of the structure and variability of NORs in Anura. Chromosoma 87: 327–344.

    Google Scholar 

  • Schweizer, D. 1976. Reverse fluorescent chromosome banding with Chromomycin and DAPI. Chromosoma 58: 307–324.

    Google Scholar 

  • Solari, J.A., M.H. Thorne, B.L. Sheldon & C.B Gillies, 1991. Synaptonemal complexes of triploid (ZZW) chickens: Z-Z pairing predominates over Z-W pairing. Genome 34: 718–726.

    Google Scholar 

  • Venere, P.C. & P.M. Galetti Jr., 1985. Natural triploidy and chromosome B in fish Curimata modesta (Curimatidae, Characiformes). Rev. Bras. Genet. VIII(4): 681–687.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Cell Biology and Genetics, State University of Maringá, 87020-900, Maringá, PR, Brazil; (Fax

    Luciana Andreia Borin & Isabel Cristina Martins-Santos

  2. Department of Morphology, Institute of Life Science, Paulista State University, Botucatu, SP, Brazil

    Claudio Oliveira

Authors
  1. Luciana Andreia Borin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Isabel Cristina Martins-Santos
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Claudio Oliveira
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Borin, L.A., Martins-Santos, I.C. & Oliveira, C. A Natural Triploid in Trichomycterus Davisi (Siluriformes, Trichomycteridae): Mitotic and Meiotic Characterization by Chromosome Banding and Synaptonemal Complex Analyses. Genetica 115, 253–258 (2002). https://doi.org/10.1023/A:1020667526552

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1020667526552

Search

Navigation