Abstract
The study of the accumulation pattern of extracellular proteins with chitinase activity in the parent Serratia marcescens strain Bú 211 (ATCC 9986) grown in the presence of mitomycin C and its mutant strain with the constitutive synthesis of chitinases grown in the absence of the inducer showed that chitinase activity appeared in the culture liquids of both strains at the end of the exponential phase (4 h of growth) and reached a maximum in the stationary phase (18–20 h of growth). The analysis of the culture liquids (12 h of growth) by denaturing electrophoresis in PAAG followed by the protein renaturation step revealed the presence of four extracellular proteins with chitinase activity and molecular masses of 21, 38, 52, and 58 kDa.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
REFERENCES
Feofilova, E.P., Kletochnaya stenka gribov (Fungal Cell Wall), Moscow: Nauka, 1983.
Materialy konferentsii “Novye perspektivy v issledovanii khitina i khitozana” (Proc. Conf. “New Prospects in the Investigation of Chitin and Chitosan”), Moscow: VNIRO, 1999.
Monreal, J. and Reese, E., The Chitinase of Serratia marcescens, Can. J. Microbiol., 1969, vol. 15, no. 7, pp. 689–696.
Porfir'eva, O.V., Yusupova, D.V., Zotkina, N.L., Sokolova, R.B., and Gabdrakhmanova, L.A., Chitinolytic Complex of Serratia marcescens and Peculiarities of Its Biosynthesis, Mikrobiologiya, 1997, vol. 66, no. 3, pp. 347–358.
Yusupova, D.V., Porfir'eva, O.V., Sokolova, R.B., Ponomareva, A.Z., and Gabdrakhmanova, L.A., The Physiological and Biochemical Characteristics of the Serratia marcescens Strain with Enhanced Chitinase Activity, Biotechnologiya, 1997, no. 2, pp. 3–9.
Yusupova, D.V., Sokolova, R.B., Porfir'eva, O.V., and Ponomareva, A.Z., The Induction of the Synthesis of the Extracellular Serratia marcescens Endonuclease by Agents Suppressing DNA Replication, Mikrobiologiya, 1991, vol. 60, no. 2, pp. 279–284.
Chigaleichik, A.G. and Pirieva, D.A., The Extracellular Chitinase of Aeromonas liquefaciens, Prikl. Biokhim. Mikrobiol., 1976, vol. 12, no. 2, pp. 238–242.
Chigaleichik, A.G., Pirieva, D.A., and Rylkin, S.S., The Chitinase of Serratia marcescens VKM B-851, Prikl. Biokhim. Mikrobiol., 1976, vol. 12, no. 4, pp. 581–586.
Yamada, H. and Imoto, T.A., Convenient Synthesis of Glycol Chitin, a Substrate of Lysozyme, Carbohydr. Res., 1981, vol. 92, pp. 160–162.
Laemmli, U.K., Cleavage of Structural Proteins during Assembly of the Head of Bacteriophage T4, Nature (London), 1970, vol. 227, no. 5259, pp. 680–685.
Trudel, J. and Asselin, A., Detection of Chitinase Activity after Polyacrylamide Gel Electrophoresis, Anal. Biochem., 1989, vol. 178, no. 2, pp. 362–366.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Yusupova, D.V., Petukhova, E.V., Sokolova, R.B. et al. The Accumulation of Proteins with Chitinase Activity in the Culture Liquids of the Parent and Mutant Serratia marcescens Strains Grown in the Presence of Mitomycin C. Microbiology 71, 546–549 (2002). https://doi.org/10.1023/A:1020554618822
Issue Date:
DOI: https://doi.org/10.1023/A:1020554618822