Abstract
Somatic embryogenesis was achieved directly from pseudo-bulblettransverse thin cell layers (tTCL) of Lilium longiflorum.Embryo-like structures (globular embryos) were obtained from different sizeexplants of pseudo-bulblet tTCLs after 45 days culture on Murashige and Skoog,1962 (MS) medium containing 5.4 μM naphthalene acetic acid(NAA)and 1.1 μM thidiazuron (TDZ). The embryo-like structures werethen isolated and mass proliferated on MS medium, containing 5.4μM NAA and 0.4 μM TDZ, every 45 days. A0.8–1.0 mm thick explant was shown to be optimal forobtaining the highest number of embryo-like structures. For plant regenerationthese structures were transferred to hormone-free MS medium with 30g/l sucrose. All of these structures formedplantlets after 90 days culture.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Beyl C.A. and Sharma G.C. 1983. Picloram induced somatic embryogenensis in Gasteria and Haworthia. Plant Cell Tiss. Org. Cult. 2: 123–132.
Bui V.L., Nhut D.T. and Tran Thanh Van K. 1999. Plant production via shoot regeneration from thin cell layer pseudo-bulblet explants of Lilium longiflorum in vitro. C. R. Acad. Sci. Paris 322: 303–310.
Duncan D.B. 1995. Multiple range and multiple F test. Biometrics 11: 1–42.
Famaelaer I., Ennik E., Van Tuyl J.M., Meijer H. and Creemers-Molenaar C. 1996. The establishment of suspension and meristem cultures for the development of a protoplasts and fusion in lily. Acta. Hort. 414: 161–168.
Haensch K.T. 1996. Plant regeneration through somatic embryogenesis in different genotypes of Lilium hybrids. Gartenbauw 61: 214–218.
Loffler H.J.M., Mouris J.R. and Van Harmelen M.J. 1990. In vitro selection for resistance against Fusarium oxysporum in lily: prospects. Lily Yearb. N. Am. Lily Soc.
Mizuguchi S., Ohkawa M. and Ikekawa F. 1994. Effect of naphthaleneacetic acid and benzyladenine on the growth of white callus and formation of bulblets from callus induced from mother-scale of Lilium japonicum Thunb. J. Soc. Hort. Sci. 63: 131–137.
Murashige T. and Skoog F. 1962. A revised medium for a rapid growth and bioassay with tobacco tissue culture. Physiol. Plant 15: 473–479.
Nhut D.T. 1998. Micropropagation of lily (Lilium longiflorum) via in vitro stem node and pseudo-bulblet culture. Plant Cell Rep. 17: 913–916.
Nhut D.T., Bui V.L., Tanaka M. and Tran Thanh Van K. 2001. Shoot induction and plant regeneration from receptacle tissue of Lilium longiflorum. Sci. Hort. 87: 131–138.
Nhut D.T., Bui V.L. and Tran Thanh Van K. 2001. Manipulation of the morphogenetic pathways of Lilium longiflorum transverse thin cell layer explants by auxin and cytokinin. In Vitro Cell Bio. Dev. 37: 44–49.
Nhut D.T., Bui V.L., Fukai S., Tanaka M. and Tran Thanh Van K. 2001. Effects of activated charcoal, explant size, explant position and sucrose concentration on plant and shoot regeneration of Lilium longiflorum via young stem culture. Plant Growth Reg. 33: 59–65.
Saiju H.K., Rajbhandary S.B. and Malla S.B. 1994. In vivo rooting of in vitro produced microshoots of Lilium longiflorum. Abstracts of VIIIth Intern. Congress of Plant Tissue and Cell culture, Firenze, June 12-17, Abstract S1–125.
Tribulato A., Remotti P.C., Loffler H.J.M. and Van Tuyl J.M. 1997. Somatic embryogenesis and plant regeneration in Lilium longi-florum Thumb. Plant Cell Rep. 17: 113–118.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Nhut, D.T., Van Le, B., Minh, N.T. et al. Somatic embryogenesis through pseudo-bulblet transverse thin cell layer of Lilium longiflorum . Plant Growth Regulation 37, 193–198 (2002). https://doi.org/10.1023/A:1020532511081
Issue Date:
DOI: https://doi.org/10.1023/A:1020532511081