Abstract
A simple, rapid, and sensitive procedure for the identification of plant extracellular α- and β-galactosidase is described using callus cultures of seedlings from the tested plant, roots of 4-6 day old seedlings of Californian poppy germinating on agar plates, and cell suspension cultures cultivated from callus cultures. Synthetic substrates were used for the determination of the intra- and extracellular activities of α- and β-galactosidase. Many imino- or azasugars are strong glycosidase inhibitors, and some of them show promising chemotherapeutic effects against viral disease and are potential antidiabetic as well as antitumor agents. This fact aroused our interest for a rapid and sensitive assay method of the activity of α- and β-galactosidases of plant origin. The results presented here show the perspective usefulness of extracellular galactosidases of plant origin in inhibitory and/or biotechnological studies.
Similar content being viewed by others
REFERENCES
T. Chrzaszcz and J. Janicki, Biochem. 260, 354 (1993).
J. Arends and B. H. L. Willms, Horm. Metab. Res. 18, 761 (1986).
B. L. Rhinhart, M. E. Begovic, and K. M. Robinson, Biochem. Pharmacol., 41, 223 (1991).
M. E. Grace, P. N. Graves, F. I. Smith, and G. A. Grabowski, J. Biol. Chem. 265, 6827 (1990).
R. J. Nash, K. A. Fenton, A. M. R. Gatehouse, and E. A. Bell, Entomol. Exp. Appl., 42, 71 (1986).
S. Schlesinger, A. H. Koyama, C. Malfer, S. L. Gee, and M. J. Schlesinger, Virus Res. 2, 139 (1985).
N. Asano, H. Kizu, K. Oseki, E. Tomioka, K. Matsui, M. Okamoto, and M. Baba, J. Med. Chem. 38, 2349 (1995).
N. Asano, M. Nishida, M. Miyauchi, K. Ikeda, M. Yamamoto, H. Kizu, Y. Kameda, A. A. Watson, R. J. Nash, and G. W. J. Fleek, Phytochemistry, 53, 379 (2000).
E. S. ElAshry, N. Rashed, A. H. S. Shobier, Part 1. Pharmazie 55, 251 (2000).
E. S. ElAshry, N. Rashed, A. H. S. Shobier, Part 2. Pharmazie 55, 331 (2000).
E. S. ElAshry, N. Rashed, A. H. S. Shobier, Part 3. Pharmazie 55, 403 (2000).
M. Sekimata, K. Ogura, Y. Tsumuraya, Y. Nashimoto, and S. Yamamoto, Plant. Physiol., 90, 567 (1989).
J. Poor, Biol. Plant. 40, 161 (1997/98).
P. Mucaji, D. Grancai, M. Nagy, Z. Visnovska, and K. Ubik, Czech. Slov. Pharm., 49, 75 (2000).
E. Eisenreichova, M. Haladova, P. Mucaji, M. Budesinsky, and K. Ubik, Pharmazie, 55, 549 (2000).
A. Vachalkova, E. Eisenreichova, M. Haladova, P. Mucaji, B. Jozova, and L. Novotny, Neoplasma, 47, 313 (2000).
N. S. Paek, O. J. Kanag, H. S. Lee, J. J. Lee, J. J. Choi, T. M. Kim, and J. J. Kim, Biosci. Biotechnol. Biochem., 62, 588 (1998).
J. Stano, P. Nemec, L. Bezakova, P. Kovacs, D. Kakoniova, K. Neubert, and D. Liskova, Pharmazie 52, 242 (1997).
F. Andriamainty, J. Stano, K. Micieta, A. Barth, H. Barthova, J. Cizmarik, and M. Korenova, Hort. Sci. 27, 131 (2000).
B. Machova, Diploma Thesis, Faculty of Pharmacy, Comenius University, Bratislava, 1994.
P. J. Stoward and A. G. E. Pearse, Histochemistry. A laboratory manual., Churchill Livingstone, Edinburgh, London, Melbourne, New York, Tokyo, (1991).
J. Poor, J. Stano, K. Neubert, J. Cizmarik, F. H. Andriamainty, and N. Borovkov, Bull. Food Res. 37, 33 (1998).
M. M. Bradford, Anal. Biochem., 72, 248 (1976).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Micieta, K., Tokhtaeva, E., Stano, J. et al. A Simple and Rapid Method of Identification of Extracellular Plant Galactosidases. Chemistry of Natural Compounds 38, 284–287 (2002). https://doi.org/10.1023/A:1020444430614
Issue Date:
DOI: https://doi.org/10.1023/A:1020444430614