Abstract
Development of an efficient in vitro plantlet regeneration system from tubers of Stachys sieboldii (Miq.), a traditional Chinese medicinal plant and vegetable, is described. Adventitious shoots with an average of 9.1 (after 4 weeks of incubation) shoots per explant were obtained from 60.4% of tuber explants cultured on Murashige and Skoog (MS) medium containing 1.0 mg l−1 thidiazuron (TDZ), and after another 4 weeks of incubation on growth regulator free MS medium, 33.5 shoots per explant were achieved. Subcultures showed results that were similar to those of the initial culture. Excised shoots were rooted both on MS and on half strength MS medium with a frequency of about 95%. Excised shoots were also rooted ex vitro by directly planting them into cups containing sand. Regenerated plants with well developed shoots and roots were successfully transplanted to soil, after which they developed free of abnormalities.
Similar content being viewed by others
References
Instituto Botanico Boreali-Occidentali Academiae Sinicae Edita (1983) Flora Tsinlingensis. Science Press, Beijing 1(4): 256–257
Hare PD & van Staden J (1994) Inhibitory effect of thidiazuron on the activity of cytokinin oxidase isolated from soybean callus. Plant Cell Physiol. 35: 1121–1125
Hayashi K, Nagamatsu T, Ito M, Yagita H & Suzuki Y (1996) Acteoside, a component of Stachys sieboldii MIQ, may be a promising antinephritic agent (3): effect of acteoside on expression of intercellular adhesion molecule-1 in experimental nephritic glomeruli in rats and cultured endothelial cells. Jpn J. Pharmacol. 70(2): 157–68
Kaneda Y, Tabei Y, Nishimura K, Akihama T & Kitamura (1997) Combination of thidiazuron and basal media with low salt concentrations increases the frequency of shoot organogensis in soybeans [Glycine max (L.) merr.]. Plant Cell Rep. 17: 8–12
Khussein IA, Kochneva EZ & Khadeeva NV (2000) Change in the spectra of peroxidases in regenerants of Stachys sieboldii (Miq.) as a result of hormonal and mutagenic effects. Genetika 36(8): 1093–1099
Meane LM & Debergh PC (1983) Rooting of tissue cultured plants under in vivo conditions. Acta Hort. 131: 201–208
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol. Plant 15: 473–497
Nirmal Babu K, Anu A, Remashree AB & Praveen (2000) Miropropagation of Curry leaf tree. Plant Cell Tiss. Org. Cult. 61: 199–203
Yamahara J, Kitani T, Kobayashi H & Kawahara Y (1990) Studies on Stachys sieboldii MIQ. II. Antianoxia action and the active constituents. Journal of the Pharmaceutical Society of Japan 110(12): 932–935
Yang J, Hu Z, Guo GQ & Zheng GC (2001) In vitro plant regeneration from cotyledon explants of Swainsona salsula Taubert (Saline swainsona). Plant Cell Tiss. Org. Cult. 66(1): 35–39
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Li, W., Gao, HH., Lu, R. et al. Direct plantlet regeneration from the tuber of Stachys sieboldii . Plant Cell, Tissue and Organ Culture 71, 259–262 (2002). https://doi.org/10.1023/A:1020387227982
Issue Date:
DOI: https://doi.org/10.1023/A:1020387227982