Abstract
DNA marker-assisted selection was employed to select Xa-21 bacterial blight resistance and waxy (Wx) genes. Genotypes with both genes were selected from four F2 populations involving indica × indica, indica × intermediate and japonica × indica crosses. With the assistance of PCR marker, 13 true breeding lines carrying Xa-21 were identified from F2 generation of IRBB 21 × G 11353 cross. Similarly ten, eleven and fifty two plants having Xa-21 gene were isolated from G 3005-4-1 × IRBB 21, IRBB 21 × HJX 74 and IRBB 21 × SY 2crosses respectively. The lines with Wx gene in both homozygous and heterozygous state were also scored from the above crosses. Twenty plants having both Xa-21 and Wx loci in homozygous state were identified. DNA-based progeny testing was conducted to ensure the selection of homozygous lines for Xa-21 and Wx genes. Finally,twenty true breeding lines with high amylose content and Xa-21 gene were isolated from four crosses. These homozygous lines are phenotypically superior and resistant to Chinese race 5 of the bacterial blight pathogen. Fifty-six germplasm sources were surveyed for PCR polymorphism in order to facilitate future PCR-based marker assisted transfer of bacterial blight resistance genes xa-5, xa-13 and Xa-21 to any desired varietal background which will be useful for selection of parents in breeding programmes.
Similar content being viewed by others
References
Ayres, M., A.M. McClung, P.D. Larkin, F.L. Bligh, C.A. Jones & W.D. Park, 1997. Microsatellites and a single-nucleotide polymorphism differentiate apparent amylose classes in an expended pedigree of US rice germplasm. Theor Appl Genet 94: 773–781.
Blair, M.W & S.R. McCouch, 1997. Microsatellite and sequence tagged site markers diagnostic for the rice bacterial leaf blight resistance gene xa5. Theor Appl Genet 95: 174–184.
Bligh, H.F.J., R.I. Till & C.A. Jones, 1995. A microsatellite sequence closely linked to the waxy gene of Oryza sativa. Euphytica 86: 83–85.
Chunwongse, J., G.B. Martin & S.D. Tanksley, 1993. Pre germination genotype screening using PCR amplification of half seeds. Theor Appl Genet 86: 694–698.
Gao, H.P., X.H. Lin, G.X. Yu, D.P. Zhang & Y.F. Xie, 1999. Inheritance of resistance to bacterial blight of four Yunan rice varieties. Rice Genet Newsl 12: 233–234.
Huang, N., E.R. Angeles, J. Domingo, G. Magpantay, A.S. Singh, G. Zhang, N. Kumaravadivel, J. Bennett & G.S. Khush, 1997. Pyramiding of bacterial blight resistance genes in rice: marker assisted selection using RFLP and PCR. Theor Appl Genet 95: 313–320.
Juliano, B.O., 1971. A simplified assay for milled rice amylase. Cereal Sci Today 16: 334–340.
Kangle, Z.P.K., J. Subudhi, J. Domingo, G. Magpantay & N. Huang, 1995. Rapid DNA isolation for marker assisted selection in rice breeding. Rice Genet Newsl 12: 255–258.
Kauffman, H.E., A.P.K. Reddy, S.P.Y. Hsieh & S.P. Merca, 1973. An improved technique for evaluating resistance of rice varieties to Xanthomonas oryzae. Plant Disease Rep 57: 537–541.
Kinoshita, T., 1995. Report of the committee on gene symbolization nomenclature and linkage groups, Rice Genet Newsl 12: 24–93.
McKenzie, K.S. & K.S. Rutger, 1983. Genetic analysis of amylose content, alkali spreading score and grain dimensions in rice. Crop Sci 23: 306–313.
Mew, T.W., 1989. An overview of the world bacterial bight situation. In: Bacterial Blight of Rice, pp. 7–12. International Rice Research Institute, Los Banos, Laguna, Philippines.
Mew, T.W. & C.M. Vera-Cruz, 1979. Variability of Xanthomonas oryzae in infection of rice differential. Phytopathol 69: 152–155.
Pooni, H.S., I. Kumar & G.S. Khush, 1993. Genetical control of amylose content in selected crosses of indica rice. Heredity 70: 269–280.
Ronald, P.C., B. Albato, R. Tabien, M.L.P. Abenes, K.S. Wu, S.R. McCouch & S.D. Tanksley, 1992. Genetic and physical analysis of the rice bacterial blight disease resistance locus Xa 21. Mol Gene Genet 236: 113–120.
Sano, Y., M. Kastumata & K. Okuno, 1986. Genetic studies of speciation in cultivated rice. 5. Inter-and intra-specific differentiation in the waxy gene expression in rice. Euphytica 35: 1–9.
Wang, G.L., T.E. Holstein, W.Y. Song, H.P. Wang & P.C. Ronald, 1995. Construction of a rice bacterial artificial chromosome library and identification of clones linked to the Xa-21 disease resistance locus. The Plant J 7: 525–533.
Yoshimura, S., A. Yoshimura, N. Iwata, S.R. McCouch, M.L. Abenes, M.R. Baraoidan, T.W. Mew & R.J. Nelson, 1995. Tagging and combining bacterial blight resistance genes in rice using RAPD and RFLP markers. Mol Breeding 1: 375–387.
Zhang, G., E.R. Angeles, M.L. Abenes, G.S. Khush & N. Huang, 1996. RAPD and RFLP mapping for the bacterial resistance gene xa-13 in rice. Theor Appl Genet 93: 65–70.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ramalingam, J., Basharat, H. & Zhang, G. STS and microsatellite marker-assisted selection for bacterial blight resistance and waxy genes in rice, Oryza sativa L.. Euphytica 127, 255–260 (2002). https://doi.org/10.1023/A:1020267000418
Issue Date:
DOI: https://doi.org/10.1023/A:1020267000418