Skip to main content
SpringerLink
Log in

Mannosylphosphodolichol Synthase Activity is Associated with a 32 kDa Phosphoprotein

  • Published:
Bioscience Reports

Abstract

Failure of actinomycin D to block the activation of Dol-P-Man synthase in isoproterenol-treated capillary endothelial cells, supported that isoproterenol effect was not mediated by active transcription of the Dol-P-Man synthase gene during a short-term β-adrenoreceptor stimulation. Instead, it was a net effect of protein phosphorylation by cAMP-dependent protein kinase. Using antibody as a probe we have now demonstrated that Dol-P-Man synthase activity is associated with a 32 kDa ER phosphoprotein.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

REFERENCES

  1. Kornfeld, R. and Kornfeld, S. (1985) Ann. Rev. Biochem. 54:631-664.

    Google Scholar 

  2. Chapman, A., Trowbridge, I. S., Hyman, R., and Kornfeld, S. (1979) Cell 17:509-515.

    Google Scholar 

  3. Banerjee, D. K., Scher, M. G., and Wacchter, C. J. (1981) Biochemistry 20:1561-1568.

    Google Scholar 

  4. Tanner, W. and Lehle, L. (1987) Biochim. Biophys. Acta 906:81-99.

    Google Scholar 

  5. Orlean, P. (1990) Mol. Cellular Biol. 10:5796-5806.

    Google Scholar 

  6. Banerjee, D. K., Kousvelari, E. E., and Baum, B. J. (1985) Biochem. Biophys. Res. Commun. 126:123-129.

    Google Scholar 

  7. Banerjee, D. K., Kousvelari, E. E., and Baum, B. J. (1987) Proc. Natl. Acad. Sci. (USA) 84:6389-6393.

    Google Scholar 

  8. Banerjee, D. K., Martinez, J. A., Schutzbach, J. S., and Hughey, P. (1996) FASEB J. 10:A1107.

    Google Scholar 

  9. Banerjee, D. K., Ornberg, R. L., Youdim, M. G. H., Heldman, E., and Pollard, H. B. (1985) Proc. Natl. Acad. Sci. (USA) 82:4702-4706.

    Google Scholar 

  10. Banerjee, D. K. (1989) J. Biol. Chem. 264:2024-2028.

    Google Scholar 

  11. Bradford, M. M. (1976) Analyt. Biochem. 72:248-254.

    Google Scholar 

  12. Laemmli, U. K. (1970) Nature 227:680-685.

    Google Scholar 

  13. Bonner, W. M. and Laskey, R. A. (1974) Eur. J. Biochem. 46:83-88.

    Google Scholar 

  14. Chatterjee, S., Ghosh, N., and Khurana, S. (1992) J. Biol. Chem. 267:7145-7148.

    Google Scholar 

  15. Das, S. K., Mukherjee, S., and Banerjee, D. K. (1994) Mol. Cell. Biochem. 146:49-54.

    Google Scholar 

  16. Taylor, S. S., Bucchler, J. A., and Yonemoto, W. (1990) Ann. Rev. Biochem. 59:971-1005.

    Google Scholar 

  17. Banerjee, D. K. and Vendrell-Ramos, M. (1993) Indian J. Biochem. Biophys. 30:389-394.

    Google Scholar 

  18. Haselback, E. G. and Tanner, W. (1982) Proc. Natl. Acad. Sci. (USA) 79:1520-1524.

    Google Scholar 

  19. Orlean, P., Albright, C., and Robbins, P. W. (1988) J. Biol. Chem. 263:17499-17507.

    Google Scholar 

  20. Mazhari-Tabrizi, R., et al., (1996) Biochem. J. (London) 316:853-858.

    Google Scholar 

  21. Zimmerman, J. W., Specht, C. A., Ceganes, B. X., and Robbins, P. W. (1996) Yeast 12:765-771.

    Google Scholar 

  22. Colusi, P. A., Taron, C. H., Mack, J. C., and Orlean, P. (1997) Proc. Natl. Acad. Sci. (USA) 94:7873-7878.

    Google Scholar 

  23. Tomita, S., Inoue, N., Maeda, Y., Ohishi, K., Takeda, J., and Kinoshita, T. (1998) J. Biol. Chem. 273:9249-9254.

    Google Scholar 

  24. Krusezewska, J., Palamarczyk, G., and Kubicek, C. P. (1991) FEMS Microbiol. Letts. 80:81-86.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biochemistry, School of Medicine, University of Puerto Rico, San Juan, PR, 00936-5067, USA

    Dipak K. Banerjee, Jaime J. Dasilva & Betzaida Bigio

Authors
  1. Dipak K. Banerjee
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jaime J. Dasilva
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Betzaida Bigio
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Dipak K. Banerjee.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Banerjee, D.K., Dasilva, J.J. & Bigio, B. Mannosylphosphodolichol Synthase Activity is Associated with a 32 kDa Phosphoprotein. Biosci Rep 19, 169–177 (1999). https://doi.org/10.1023/A:1020221602373

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1020221602373

Search

Navigation