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Post-translational modification of barley 14-3-3A is isoform-specific and involves removal of the hypervariable C-terminus

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Abstract

The 14-3-3 protein family is a family of regulatory proteins involved in diverse cellular processes. In a previous study of regulation of individual 14-3-3 isoforms in the germinating barley embryo, we found that a post-translationally modified, 28 kDa form of 14-3-3A was present in specific cell fractions of the germinated embryo. In the present study, we identify the nature of the modification of 14-3-3A, and show that the 28 kDa doublet is the result of cleavage of the C-terminus. The 28 kDa forms of 14-3-3A lack ten or twelve amino acid residues at the non-conserved C-terminus of the protein, respectively. Barley 14-3-3B and 14-3-3C are not modified in a similar way. Like the 30 kDa form, in vitro produced 28 kDa 14-3-3A is still capable of binding AHA2 H+-ATPase in an overlay assay. Our results show a novel isoform-specific post-translational modification of 14-3-3 proteins that is regulated in a tissue-specific and developmental way.

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Author notes

  1. Christa Testerink

    Present address: Department of Plant Physiology, Swammerdam Institute for Life Sciences, University of Amsterdam, Kruislaan 318, 1098, KW Amsterdam, Netherlands

Authors and Affiliations

  1. Centre for Phytotechnology, Leiden University/Netherlands Organization for Applied Scientific Research (UL/TNO), TNO Department of Applied Plant Sciences, Wassenaarseweg 64, 2333 AL, Leiden, Netherlands

    Christa Testerink, Mieke J. van Zeijl, Jan W. Kijne & Mei Wang

  2. Plant Physiology and Anatomy Laboratory, Department of Plant Biology, Royal Veterinary and Agricultural University, Thorvaldsensvej 40, 1871 , Frederiksberg C, Copenhagen, Denmark

    Katrine Drumm & Michael G. Palmgren

  3. Section for Plant Pathology, Department of Plant Biology, Royal Veterinary and Agricultural University, Thorvaldsensvej 40, 1871 , Frederiksberg C, Copenhagen, Denmark

    David B. Collinge

Authors
  1. Christa Testerink
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  2. Mieke J. van Zeijl
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  3. Katrine Drumm
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  7. Mei Wang
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Testerink, C., van Zeijl, M.J., Drumm, K. et al. Post-translational modification of barley 14-3-3A is isoform-specific and involves removal of the hypervariable C-terminus. Plant Mol Biol 50, 535–542 (2002). https://doi.org/10.1023/A:1019869900285

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