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Genome-wide identification of 14-3-3 gene family and characterization of their expression in developmental stages of Solanum tuberosum under multiple biotic and abiotic stress conditions

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Abstract

GF14 proteins are a family of conserved proteins involved in many cellular processes including transport, growth, metabolism, and stress response. However, only few reports are available regarding the 14-3-3 genes in potato. In this study, twelve 14-3-3 genes were detected in the potato genome. Based on their phylogenetic relationships, the StGF14 family members were categorized into two classes. Gene expression analysis demonstrated that StGF14h, StGF14a, and StGF14k had the highest gene expression, induced by abiotic and biotic stresses in all three tissues. The number of exons in 14-3-3 genes ranged from four to seven and most of these genes in the same subfamily had similar exon-intron patterns. The results of our study showed that the conserved motifs are similar in most of the proteins in each group. The intron-exon patterns and the composition of conserved motifs validated the 14-3-3 gene phylogenetic classification. According to the genome distribution results, 14-3-3 genes were located unevenly on the 12 Solanum tuberosum chromosomes. We find out 97 orthologous gene pairs between potato and Arabidopsis as well as 15 paralogous genes among potato genomes. Our results showed that GF-14 genes have an effective role in functional and molecular mechanisms in response to environmental stresses.

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Acknowledgements

The authors are grateful for receiving assistance from the Shahid Beheshti University, Tehran, Iran.

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  1. Department of Plant Sciences and Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran

    Zahra Hajibarat, Abbas Saidi & Zohreh Hajibarat

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  1. Zahra Hajibarat
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  2. Abbas Saidi
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  3. Zohreh Hajibarat
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ZH helped in carrying out the experiment and writing the MS. AS helped in analysis and editing of the manuscript. ZH helped in writing of the manuscript. All authors have read and approved the manuscript.

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Correspondence to Abbas Saidi.

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Hajibarat, Z., Saidi, A. & Hajibarat, Z. Genome-wide identification of 14-3-3 gene family and characterization of their expression in developmental stages of Solanum tuberosum under multiple biotic and abiotic stress conditions. Funct Integr Genomics 22, 1377–1390 (2022). https://doi.org/10.1007/s10142-022-00895-z

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