Abstract
Site-directed mutagenesis was used to study the structural basis of color diversity of fluorescent proteins by the example of two closely related proteins from one organism (coral polyp Zoanthus sp.), one of which produces green and the other, yellow fluorescence. As a result, the following conversions of emission colors were performed: from yellow to green, from yellow to a dual color (yellow and green), and from green to yellow. The saltatory character of the spectral transitions and the manifestation of the dual-color fluorescence suggest that chemically different fluorophores are responsible for the green and yellow fluorescence. The simultaneous presence of three residues, Gly63, Lys65, and Asp68, is necessary for the efficient formation of the yellow rather than green fluorophore.
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REFERENCES
Chaflie, M., Tu, Y., Euskirchen, G., Ward, W.W., and Prasher, D.C., Science, 1994, vol. 263, pp. 802–805.
Kendall, J.M. and Badminton, M.N., Trends Biotechnol., 1998, vol. 16, pp. 216–224.
Bajno, L. and Grinstein, S.J., Immunol. Methods, 1999, vol. 232, pp. 65–75.
Pollok, B.A. and Heim, R., Trends Cell Biol., 1999, vol. 9, pp. 57–60.
White, J. and Stelzer, E., Trends Cell Biol., 1999, vol. 9, pp. 61–65.
Gonzalez, C. and Bejarano, L.A., Trends Cell Biol., 2000, vol. 10, pp. 162–165.
Margolin, W., Methods, 2000, vol. 20, pp. 62–72.
Shimomura, O., FEBS Lett., 1979, vol. 104, pp. 220–222.
Cody, C.W., Prasher, D.C., Wastler, W.M., Prendergast, F.G., and Ward, W.W., Biochemistry, 1993, vol. 32, pp. 1212–1218.
Ormo, M., Cubitt, A.B., Kallio, K., Gross, L.A., Tsien, R.Y., and Remington, S.J., Science, 1996, vol. 273, pp. 1392–1395.
Yang, F., Moss, L.G., and Phillips, G.N., Jr., Nature Biotechnol, 1996, vol. 14, pp. 1246–1251.
Palm, G.J., Zdanov, A., Gaitanaris, G.A., Stauber, R., Pavlakis, G.N., and Wlodawer, A., Nature Struct. Biol., 1997, vol. 4, pp. 361–365.
Tsien, R.Y. and Prasher, D.C., Green Fluorescent Protein, Chalfie, M. and Kain, S., Eds., New York: Willey–Liss, 1998, pp. 97–118.
Remington, S.J., Methods Enzymol., 2000, vol. 305, pp. 196–211.
Heim, R., Prasher, D.C., and Tsien, R.Y., Proc. Natl. Acad. Sci. USA, 1994, vol. 91, pp. 12 501–12 504.
Matz, M.V., Fradkov, A.F., Labas, Y.A., Savitsky, A.P., Zaraisky, A.G., Markelov, M.L., and Lukyanov, S.A., Nature Biotechnol., 1999, vol. 17, pp. 969–973.
Fradkov, A.F., Chen, Y., Ding, L., Barsova, E.V., Matz, M.V., and Lukyanov, S.A., FEBS Lett., 2000, vol. 479, pp. 127–130.
Wall, M.A., Socolich, M., and Ranganathan, R., Nature Struct. Biol., 2000, vol. 7, pp. 1133–1138.
Yarbrough, D., Wachter, R.M., Kallio, K., Matz, M.V., and Remington, S.J., Proc. Natl. Acad. Sci. USA, 2001, vol. 98, pp. 462–467.
Lukyanov, K.A., Fradkov, A.F., Gurskaya, N.G., Matz, M.V., Labas, Y.A., Savitsky, A.P., Markelov, M.L., Zaraisky, A.G., Zhao, X., Fang, Y., Tan, W., and Lukyanov, S.A., J. Biol. Chem., 2000, vol. 275, pp. 25879–25882.
Baird, G.S., Zacharias, D.A., and Tsien, R.Y., Proc. Natl. Acad. Sci. USA, 2000, vol. 97, pp. 11 984–11 989.
Terskikh, A., Fradkov, A., Ermakova, G., Zaraisky, A., Tan, P., Kaj, 2001, vol. 40, pp. 2502–2510.
Wiehler, J., von Hummel, J., and Steipe, B., FEBS Lett., 2001, vol. 487, pp. 384–389.
Mizuno, H., Sawano, A., Eli, P., Hama, H., and Miyawaki, A.,Biochemistry, 2001, vol. 40, pp. 2502–2510.
Gross, L.A., Baird, G.S., Hoffman, R.C., Baldridge, K.K., and Tsien, R.Y., Proc. Natl. Acad. Sci. USA, 2000, vol. 97, pp. 11990–11995.
Martynov, V.I., Savitsky, A.P., Martynova, N.Y., Savitsky, P.A., Lukyanov, K.A., and Lukyanov, S.A., J. Biol. Chem., 2001, vol. 276, pp. 21 012–21 016.
Ho, S.N., Hunt, H.D., Horton, R.M., Pullen, J.K., and Pease, L.R., Gene, 1989, vol. 77, pp. 51–59.
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Yanushevich, Y.G., Bulina, M.E., Gurskaya, N.G. et al. Key Amino Acid Residues Responsible for the Color of Green and Yellow Fluorescent Proteins from the Coral Polyp Zoanthus sp.. Russian Journal of Bioorganic Chemistry 28, 274–277 (2002). https://doi.org/10.1023/A:1019583522608
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DOI: https://doi.org/10.1023/A:1019583522608