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An alternative HPLC method for the quantification of floridoside in salt-stressed cultures of the red alga Grateloupia doryphora

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Abstract

The floridoside content of the marine red alga Grateloupiadoryphora was measured using a new, alternative High PerformanceLiquid Chromatography method suitable for the analysis of a large number ofsamples. This assay was to study the quantitative changes in floridosideconcentration in G. doryphora cultivated in differentsalinities for 10 days. Marked changes in floridoside concentration occurredrapidly observed in reponse to osmotic stress, providing evidence of itsosmoregulatory function. Interestingly, after a rapid decrease at low salinityand a strong increase at high salinity, the floridoside content then slowlyreturned to its initial level over the last 3 days of culture. This observationconfirms the high physiological capacity of G. doryphoratowithstand environmental variations and probably contributes to its actualproliferation along French coasts.

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Authors and Affiliations

  1. Laboratoire d'Ecophysiologie et de Biotechnologie des Halophytes et des Algues Marines, Université de Bretagne Occidentale-IUEM, Technopôle Brest-Iroise, F-29280, Plouzané, France

    Christelle Simon-Colin, Marie-Anne Bessières & Eric Deslandes

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  1. Christelle Simon-Colin
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  2. Marie-Anne Bessières
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  3. Eric Deslandes
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Simon-Colin, C., Bessières, MA. & Deslandes, E. An alternative HPLC method for the quantification of floridoside in salt-stressed cultures of the red alga Grateloupia doryphora . Journal of Applied Phycology 14, 123–127 (2002). https://doi.org/10.1023/A:1019539225950

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