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Pancreatic Fluid Hypersecretion in Rats After Acute Pancreatitis

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Abstract

Pancreatic exocrine function was examined inrats during the early stage of acute pancreatitisinduced by four subcutaneous injections of 20 mug/kgbody weight of cerulein at hourly intervals. Basalpancreatic fluid secretion at 6 hr after the first of fourcerulein injections was significantly elevated (27.6± 3.7 vs 17.4 ± 2.1 μl/30 min incontrol, P < 0.01) and further increased with time,reaching the peak level at 24 hr (105.1 ± 4.6 μl/30 min).Intravenous infusion of loxiglumide (50 mg/kg bodywt/hr), atropine (100 μg/kg body wt/hr), oranti-secretin serum did not modify the fluidhypersecretion observed at 24 hr after induction of acute pancreatitis.Loxiglumide, when given 30 min before the first ceruleininjection, markedly reduced fluid secretion, but couldnot inhibit the fluid hypersecretion when applied after the last cerulein injection. Leakage ofEvans blue dye into pancreatic juice was slightly butsignificantly increased in postpancreatitic ratscompared with that in the control rats (1.30 ±0.17 vs 0.75 ± 0.08 μg/ml, P ≤ 0.01),whereas that in the pancreas was not different from thecontrol rats. In vivo labeling with5-bromo-2-deoxyuridine showed active proliferation ofacinar and ductular cells at 6 hr. In addition, the fluid was rich inchloride (137.1 ± 2.5 at 24 hr vs 92.4 ±3.3 meq/liter in control, P < 0.01) but poor inbicarbonate concentration (39.0 ± 2.0 at 24 hr vs46.5 ± 1.9 mmol/liter in control, P < 0.01), indicating acinar cell secretion.These results indicate that pancreatic fluid secretionduring the early stage of acute pancreatitis induced bysupramaximal doses of cerulein was markedly increased not by CCK-, secretin-, orcholinergic-dependent mechanisms but probably by acinarcell proliferation.

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Authors
  1. Laszlo Czako
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  2. Mitsuyoshi Yamamoto
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  3. Makoto Otsuki
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Czako, L., Yamamoto, M. & Otsuki, M. Pancreatic Fluid Hypersecretion in Rats After Acute Pancreatitis. Dig Dis Sci 42, 265–272 (1997). https://doi.org/10.1023/A:1018893230319

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