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Chloramphenicol acetyltransferase expression of Escherichia coli is increased at 42°C

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Biotechnology Techniques

Abstract

Chloramphenicol acetyltransferase production in a soluble and active form was promoted four- to five-fold by increasing culture temperature of Escherichia coli from 37 to 42°C. Comparison of cat mRNA levels at the two temperatures did not explain differences in enzyme activity, suggesting a post-transcriptional regulation.

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Authors and Affiliations

  1. Department of Life Science, Hoseo University, Asan-Si ChungNam, 336-795, Korea

    Sung Jo Kim, Hye Young Jeon & Han Bok Kim

Authors
  1. Sung Jo Kim
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  2. Hye Young Jeon
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  3. Han Bok Kim
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Jo Kim, S., Young Jeon, H. & Bok Kim, H. Chloramphenicol acetyltransferase expression of Escherichia coli is increased at 42°C. Biotechnology Techniques 11, 435–438 (1997). https://doi.org/10.1023/A:1018481125858

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