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On-line monitoring of mammalian cell and yeast fermentations with a commercial biochemical analyzer

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Biotechnology Techniques

Abstract

A commercial analyzer was tested for on-line monitoring of fermentations. A new sample block was constructed to effectively degas the fermentation broth. The robust analyzer accurately measured glucose up to 110 mmol/l and lactate up to 21 mmol/l at a frequency of 1 measurement per 2 minutes directly in suspensions of mammalian and yeast cells.

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Authors and Affiliations

  1. Kluyver Laboratory for Biotechnology, Technical University Delft, Julianalaan 67, 2628BC, Delft, the Netherlands

    N. Vriezen & J.P. van Dijken

Authors
  1. N. Vriezen
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  2. J.P. van Dijken
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Vriezen, N., van Dijken, J. On-line monitoring of mammalian cell and yeast fermentations with a commercial biochemical analyzer. Biotechnology Techniques 11, 427–430 (1997). https://doi.org/10.1023/A:1018477024950

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  • DOI: https://doi.org/10.1023/A:1018477024950

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