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Construction of a green-fluorescent protein-based, insertion-inactivation shuttle vector for lactic acid bacteria and Escherichia coli

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Abstract

A shuttle vector, p5aGFP2201a, for lactic acid bacteria and E. coli was constructed by using the gene of a jellyfish green fluorescent protein ( gfp) as a selection marker. The plasmid was shown to function as a shuttle vector by its ability to carry and express a staphylococcal chloramphenicol acetyltransferase ( cat) gene into targeted hosts.

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Authors and Affiliations

  1. U.S. Department of Agriculture, ARS, Eastern Regional Research Center, 600 East Mermaid Lane, Wyndmoor, PA, 19038, USA

    D.K.Y. Solaiman & G.A. Somkuti

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  1. D.K.Y. Solaiman
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  2. G.A. Somkuti
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Solaiman, D., Somkuti, G. Construction of a green-fluorescent protein-based, insertion-inactivation shuttle vector for lactic acid bacteria and Escherichia coli. Biotechnology Letters 19, 1175–1179 (1997). https://doi.org/10.1023/A:1018473501442

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  • DOI: https://doi.org/10.1023/A:1018473501442

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