Biotechnology Techniques

, Volume 11, Issue 6, pp 413–416 | Cite as

Recovery and purification of the lipopeptide biosurfactant of Bacillus subtilis by ultrafiltration

  • Sung-Chyr Lin
  • Horng-Jyh Jiang


Surfactin, a lipopeptide biosurfactant produced as micelles by Bacillus subtilis, was recovered from the fermentation broth by ultrafiltration with a 30 kDa MWCO membrane. The retained surfactin micelles were then ruptured and collected in the permeate by adding methanol to 50% (v/v). The final yield of surfactin was 95%.


Methanol Purification Fermentation Bacillus Bacillus Subtilis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Arima, K., Kakinuma, A. and Tamura, A. (1968). Biochem. Biophys. Res. Commun. 31, 488–494.Google Scholar
  2. Cooper, D.G., MacDonald, C.R., Duff, S.J.B. and Kosaric, N. (1981). Appl. Environ. Microbiol. 42, 408–412.Google Scholar
  3. Horowitz, S. and Griffin, W.M. (1991). J. Ind. Microbiol. 7, 45–52.Google Scholar
  4. Lin, S.C., Minton, M.A., Sharma, M.M. and Georgiou, G. (1994). Appl. Environ. Microbiol. 60, 31–38.Google Scholar
  5. Mulligan, C.N. and Gibbs, B.F. (1990). J. Chem. Tech. Biotechnol. 47, 23–29.Google Scholar
  6. Peypoux, P. and Michel, G. (1992). Appl. Microbial. Biotechnol. 36, 515–517.Google Scholar
  7. Wu, C.H. and Lin, S.C. (1996). J. Chin. Colloid Interface Soc. 19, 125–136.Google Scholar

Copyright information

© Chapman and Hall 1997

Authors and Affiliations

  • Sung-Chyr Lin
    • 1
  • Horng-Jyh Jiang
    • 1
  1. 1.Department of Chemical EngineeringNational Chung Hsing UniversityTaichungTaiwan, R.O.C

Personalised recommendations