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Recovery and purification of the lipopeptide biosurfactant of Bacillus subtilis by ultrafiltration

Biotechnology Techniques

Abstract

Surfactin, a lipopeptide biosurfactant produced as micelles by Bacillus subtilis, was recovered from the fermentation broth by ultrafiltration with a 30 kDa MWCO membrane. The retained surfactin micelles were then ruptured and collected in the permeate by adding methanol to 50% (v/v). The final yield of surfactin was 95%.

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Lin, SC., Jiang, HJ. Recovery and purification of the lipopeptide biosurfactant of Bacillus subtilis by ultrafiltration. Biotechnology Techniques 11, 413–416 (1997). https://doi.org/10.1023/A:1018468723132

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  • DOI: https://doi.org/10.1023/A:1018468723132

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