Recovery and purification of the lipopeptide biosurfactant of Bacillus subtilis by ultrafiltration
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Surfactin, a lipopeptide biosurfactant produced as micelles by Bacillus subtilis, was recovered from the fermentation broth by ultrafiltration with a 30 kDa MWCO membrane. The retained surfactin micelles were then ruptured and collected in the permeate by adding methanol to 50% (v/v). The final yield of surfactin was 95%.
KeywordsMethanol Purification Fermentation Bacillus Bacillus Subtilis
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