Abstract
Alu-PRINS was successful in providing positive identification of euchromatin when oligoprimers were used at either extremity of the consensus Alu sequence. This technique was sensitive, as shown by the ability to detect small regions of euchromatin translocated to the short arm of an acrocentric chromosome. Since the Alu-PRINS technique is rapid and relatively simple, it will be useful in the diagnostic cytogenetics laboratory for the assessment of aberrant short arms of acrocentric satellites. This technique was also useful in defining the structure of small accessory marker chromosomes.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Callen DF, Ringenbergs ML, Fowler JCS, Freemantle CJ, Haan EA (1990) Small marker chromosomes in man: origin from pericentric heterochromatin of chromosomes 1, 9, and 16. J MedGenet 27: 155–159.
Callen DF, Eyre HJ, Ringenbergs ML et al. (1991) Chromosomal origin of small ring marker chromosomes in man: characterization by molecular genetics. Am J Hum Genet 48: 769–782.
Callen DF, Eyre H, Yip M-Y, Freemantle J, Haan EA (1992) Molecular cytogenetics and clinical studies of 42 patients with marker chromosomes. Am J Med Genet 43: 709–715.
Fang YY, Eyre HJ, Bohlander SK et al. (1995) Mechanisms of small ring formation suggested by the molecular characterization of two small accessory ring chromosomes derived from chromosome 4. Am J Hum Genet 57: 1137–1142.
Flint J, Wilkie AOM, Buckle VJ et al. (1995) The detection of subtelomeric chromosomal rearrangements in idiopathic mental retardation. Nature Genet 9: 132–138.
Gosden J, Hanratty D, Starling J et al. (1991) Oligonucleotide-primed in situ DNA synthesis (PRINS): a method for chromosome mapping, banding, and investigation of sequence organization. Cytogenet Cell Genet 57: 100–104.
Hindkjaer J, Koch J, Mogensen J, Kolvraa S, Bolund L (1994) Primed in situ (PRINS) labeling of DNA. Methods Mol Biol 33: 95–107.
Koch JE, Kolvraa S, Petersen KB, Gregersen N, Bolund L (1989) Oligonucleotide-priming methods for the chromosome specific labelling of alpha satellite DNA in situ. Chromosoma 98: 259–265.
Koch J, Hindkjaer J, Kolvraa S, Bolund L (1995) Construction of a panel of chromosome-specific oligonucleotide probes (PRINS-primers) useful for the identification of individual human chromosomes in situ. Cytogenet Cell Genet 71:142–147.
Pellestor F, Girardet A, Lefort G, Andre´o B, Charlieu JP (1995) Use of the primed in situ labelling (PRINS) technique for a rapid detection of chromosomes 13, 16, 18, 21, X and Y. Hum Genet 95: 12–17.
Tagle DA, Collins FS (1992) An optimized Alu-PCR primer pair for human-specific amplification of YACs and somatic cell hybrids. Hum Mol Genet 1: 121–122.
Willard C, Nguyen HT, Schmid CW (1987) Existence of at least three distinct Alu subfamilies. J Mol Evol 26: 180–186.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Callen, D., Yip, MY. & Eyre, H. Rapid detection of euchromatin by Alu-PRINS: use in clinical cytogenetics. Chromosome Res 5, 81–85 (1997). https://doi.org/10.1023/A:1018457806004
Issue Date:
DOI: https://doi.org/10.1023/A:1018457806004