Abstract
Kinetics of nisin production have been investigated in terms of endogenous features of the producer organism, Lactococcus lactis. Nisin-producing transposons (Tn Nip) were transferred to different hosts by conjugation. Constructs were cultivated in batch cultures and nisin produced was measured. The proteinase function of C2Prt (Tn Nip)-1 was eliminated by plasmid curing, resulting in the construct C2Prt - (Tn Nip)-1. C2Prt - (Tn Nip)-1 produced nisin to a higher concentration compared to C2Prt (Tn Nip)-1 and was able to maintain the maximum concentration till the end of cultivation. The final concentration of nisin produced was host-specific, because when different constructs carrying the same Tn Nip were cultivated they produced nisin to different concentrations. However, when the same host carried Tn Nip transposons derived from different donors the concentration of nisin produced was similar, suggesting that the two Tn Nip transposons may be similar.
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References
Alifax, R and Chevalier, R (1962). J Dairy Res 29:233–240
Anderson, DG and McKay, LL (1983). Appl Environ Microbiol 46:549–552
Chevalier, R, Fournaud, J, Lefebvre, E and Mocquot, G (1957). Ann Technol Agric 2:117–137
De Vuyst, L (1995). J Appl Bacteriol 78:28–33
Efstathiou, JD and McKay, LL (1977). J Bacteriol 130:257–265
Exterkate, FA (1976). Neth Milk Dairy J 30:95–105
Exterkate, FA (1985). J Dairy Res 68:562–571
Gross, E (1977). α,β-Unsaturated and related amino acids in peptides and proteins. In: Proteins Cross-Linking, M Friedman, ed vol B pp 131–153, New York: Plenum
Horn, N, Dodd, HM and Gasson, MJ (1991). Mol Gen Genet 228:129–135
Huggins, AR and Sandine, WE (1983). J Dairy Sci 67:1674–1679
Hurst, A (1981). Adv Appl Microbiol 27:85–123
Jarvis, B (1967). J Gen Microbiol 47:33–48
Kim, WS (1997). Lett Appl Microbiol (in press)
Kim, WS and Dunn, NW (1997). FEMS Microbiol Lett 146:285–289
Kim, WS, Hall, RJ and Dunn, NW (1997). Appl Microbiol Biotechnol (in press)
Kolstad, J and Law, BA (1985). J Appl Bacteriol 58:449–456
Law, BA and Kolstad, J (1983). Antonie van Leeuwenhoek 49:225–245
McKay, LL, Baldwin, KA and Efstathiou, JD (1976). Appl Environ Microbiol 32:45–52
Orberg, PK and Sandine, WE (1985). J Dairy Sci 68:572–580
Rice, GH, Stewart, FHC, Hillier, AJ and Jago, GR (1978). J Dairy Res 45:93–107
Steele, JL and McKay, LL (1986). Appl Environ Microbiol 51:57–64
Terzaghi, BE and Sandine, WE (1975). Appl Microbiol 29:807–813
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Kim, W., Hall, R. & Dunn, N. Host specificity of nisin production by Lactococcus lactis. Biotechnology Letters 19, 1235–1238 (1997). https://doi.org/10.1023/A:1018450223732
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DOI: https://doi.org/10.1023/A:1018450223732