Abstract
Six bovine cosmid-derived microsatellites (IDVGA53, BTA3/U6; IDVGA61, U13; IDVGA41, BTA12/U27; IDVGA32, BTA15/U19; IDVGA59, BTA26/U26 and IDVGA71, U8), previously assigned to cattle chromosomes, were FISH-mapped to river buffalo chromosomes (BBU) 6q15, 8q34, 13q15, 16q25, 23q22 and 24q13 respectively. Sequential FISH/RBA-banding allowed the precise identification of chromosomes and localization of probe-signals on chromosome bands. These localizations allowed us to assign indirectly, for the first time, six bovine syntenic groups to river buffalo chromosomes, thereby extending its physical map. The localization of IDVGA71 (bovine U8) to the marker BBU24 adds further information to resolve definitively cattle chromosome ambiguities involving cattle chromosomes 25, 27 and 29.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Barendse SM, Vaiman D, Kemp SJ, Sugimoto Y, et al. (1997) A medium-density genetic linkage map of the bovine genome. Mamm Genome 8: 21–28.
Committee for Standardized Karyotype of Bubalus bubalis (1994). Standard karyotype of the river buffalo (Bubalus bubalis L., 2n = 50). Report of the committee for the standardization of banded karyotypes of the river buffalo (L. Iannuzzi, coordinator). Cytogenet Cell Genet 67: 102–113.
Di Berardino D, Lioi MB, Iannuzzi L (1985) Identification of nucleolus organizer chromosomes in Cattle (Bos taurus L.) by sequential silver staining. RBA banding. Caryologia 38 (1): 95–102.
Eggen A, Fries R (1995). An integrated cytogenetic and meiotic map of the bovine genome. Anim Genet 26: 215–236.
Ferretti L, Urquhart BGD, Olsaker I, Harlizius B, et al. (1997) Cosmid-derived markers anchoring the bovine genetic map to the physical map. Mamm Genome 8: 29–36.
Iannuzzi L (1996) G-and R-banded prometaphase karyotypes in cattle (Bos taurus L.) Chrom Res 4: 448–456.
Iannuzzi L, Gallagher DS, Womack JE, Di Meo GP, Schelling CP, Groenen MAM (1996a). FISH mapping of the a-S2 casein gene on river buffalo and cattle chromosomes identifies a nomenclature discrepancy in the bovine karyotype. Chrom Res 4: 159–162.
Iannuzzi L, Gallagher DS, Di Meo GP, Diamond G, Bevins CL, Womack JE (1996b) High-resolution FISH mapping of bdefensin genes to river buffalo and sheep chromosomes suggests a chromosome discrepancy in cattle standard karyotypes. Cytogenet Cell Genet 75: 10–13.
Iannuzzi L, Di Meo GP, Perucatti A (1996c) Identification of nucleolus organizer chromosomes and frequency of active NORs in river buffalo (Bubalus bubalis L.). Caryologia 49: 27–34.
Iannuzzi L, Ferretti L, Di Meo GP, Perucatti A (1997) FISH mapping of bovine U21, U1 and U7 molecular markers to river buffalo chromosomes 3p, 5p and 5q. Chrom Res 5: 337–340.
ISCNDA89 (1990) International System for Cytogenetic Nomenclature of Domestic Animals (Di Berardino D, Hayes H, Fries R, Long S, eds). Cytogenet Cell Genet 53: 65–79.
Mezzelani A, Zhang Y, Redaelli L et al. (1995) Chromosomal localization and molecular characterization of 53 cosmidderived bovine microsatellites. Mamm Genome 6: 629–635.
Reading Conference (1980). Proceedings of the first international conference for the standardisation of banded karyotypes of domestic animals (Ford CE, Pollock DL, Gustavsson I, eds). Hereditas 92: 145–162.
Texas nomenclature (1996). Standardization of cattle karyotype nomenclature: report of the committee for the standardization of the cattle karyotype. (Popescu CP, Long S, Riggs P, Womack J, Schmutz S, Fries R, Gallagher D, eds). Cytogenet Cell Genet 74: 259–261.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Iannuzzi, L., Meo, G.P.D. & Ferretti, L. Six bovine cosmid-derived microsatellites mapping different syntenic groups are fluorescence in situ hybridization mapped to six river buffalo chromosomes. Chromosome Res 5, 541–543 (1997). https://doi.org/10.1023/A:1018441703221
Issue Date:
DOI: https://doi.org/10.1023/A:1018441703221