Abstract
G- and R banded cattle (Bos taurus L.) karyotypes at about 500 band level are reported and discussed in light of chromosome banding standard nomenclature discrepancies. GTG-, GBG-, RBA- and RBG-banded karyotypes were arranged according to the Reading Conference standard. The G- and R-banding patterns of small acrocentric and disputed chromosomes (25, 27, 28 and 29) are demonstrated using some bovid marker chromosomes. G- and R-banded ideogrammatic representations using only one common banding nomenclature were also performed. This study is a contribution to the construction of new and clearer standard G- and R-banded karyotypes for this important species.
Similar content being viewed by others
References
Ansari HA, Pearce PD, Maher DW, Malcolm AA, Broad TE (1993) Resolving ambiguities in the karyotype of domestic sheep (Ovis aries).Chromosoma 102: 340–347.
Committee for Standardized Karyotype ofBubalus bubalis (1994) Standard karyotype of the river buffalo (Bubalus bubalis L., 2n=50). Report of the committee for the standardization of banded karyotypes of the river buffalo (Iannuzzi L, coordinator).Cytogenet Cell Genet 67: 102–113.
Di Berardino D, Iannuzzi L (1982) Detailed description of Rbanded bovine chromosomes.J Hered 73: 434–438.
Di Berardino D, Iannuzzi L, Bettini TM, Matassino D (1981) Ag-NORs variation and banding homologies in two species of Bovidae:Bubalus bubalis andBos taurus.Can J Genet Cytol 39: 136–139.
Di Berardino D, Iannuzi L, Lioi MB (1985a) The high resolution R-banding pattern in bovine chromosomes.Cytogenet Cell Genet 39: 136–139.
Di Berardino D, Lioi MB, Iannuzzi L (1985b) Identification of nucleolus organizer chromosomes in cattle (Bos taurus L.) by sequential silver staining + RBA-banding.Caryologia 38: 95–102.
Di Berardino D, Lioi MB, Matassino D (1991) Sequential GTG-RBA banding pattern in prometaphase chromosomes of cattle (Bos taurus L.).Genet Sel Evol 23: 273–279.
Di Meo GP, Iannuzzi L, Ferrara L, Rubino R (1991) Identification of nucleolus organizer chromosomes in goat.Caryologia 44: 309–316.
Di Meo GP, Iannuzzi L, Perucatti A, Ferrara L (1993) Identification of nucleolus organizer chromosomes in sheep (Ovis aries L.) by sequential GBG/Ag-NOR and RBG/Ag-NOR techniques.Cytobios 75: 183–190.
Gallagher DS, Ryan AM, Liou LS, Sastry KN, Womack JE (1993) Somatic cell mapping of conglutinin (CGN1) to cattle syntenic group U29 and fluorescencein situ localization to chromosome 28.Mamm Genet 4: 716–719.
Gallagher DS, Schelling CP, Groenen MMA, Womack JE (1994) Confirmation that casein gene cluster resides on cattle chromosome 6.Mammal Genome 5: 524.
Hayes H, Petit E, Dutrillaux B (1991) Comparison of RBG-banded karyotypes of cattle, sheep and goats.Cytogenet Cell Genet 57: 51–55.
Hayes H, Petit E, Bouniol C, Popescu P (1993) Localization of the alpha S2-casein gene (CASAS2) to the homologous cattle, sheep and goat chromosomes 4 byin situ hydridization.Cytogenet Cell Genet 64: 281–285.
Iannuzzi L (1990) An improved characterization of cattle chromosomes by means of high-resolution G- and R-band comparison.J Hered 81: 80–83.
Iannuzzi L, Di Meo GP (1995) Chromosomal evolution in bovids: a comparison of cattle, sheep and goat G- and R-banded chromosomes and cytogenetic divergences among cattle, goat and river buffalo sex chromosomes.Chrom Res 3: 291–299.
Iannuzzi L, Gustavsson I, Di Meo GP, Ferrara L (1989) High resolution studies on late replicating segments (G+C bands) in mammalian chromosomes.Hereditas 110: 43–50.
Iannuzzi L, Di Meo GP, Perucatti A, Ferrara L (1990) A comparison of G- and R-banding patterns in cattle and river buffalo prometaphase chromosomes.Caryologia 43: 283–290.
Iannuzzi L, Gallagher DS, Lioi LS et al. (1994) Chromosomal localization of conglutinin (CGN1) gene to river buffalo by sequential RBH-banding and FISH.Hereditas 120: 283–286.
Iannuzzi L, Di Meo GP, Perucatti A, Ferrara L (1994) G- and R-banding comparison of sheep (Ovis aries L.) chromosomes.Cytogenet Cell Genet 68: 85–90.
Iannuzzi L, Gallagher DS, Womack GP, Di Meo GP, Schelling CP, Groenen MAM (1996a) FISH mapping of the α-S2 casein gene on river buffalo and cattle chromosomes identifies a nomenclature discrepancy in the bovine karyotype.Chrom Res 4: 159–162.
Iannuzzi L, Di Meo GP, Perucatti A (1996b) Identification of nucleolus organizer chromosomes and frequency of active NORs in river buffalo (Bubalus bubalis L.). Caryologia (in press).
ISCN (1985) An international system for human cytogenetic nomenclature (Harnden DG, Klinger HP, eds). Published in collaboration withCytogenet Cell Genet (Karger, Basle 1985). Also inBirth Defects: Original Article Series, Vol 21, no. 1, March of Dimes Birth Defects Foundation, New York, 1985.
ISCNDA 1989 (1990) International system for cytogenetic nomenclature of domestic animals (Di Berardino D, Hayes H, Fries R, Long S, eds).Cytogenet Cell Genet 53: 65–79.
Lin CC, Newton DR, Church RB (1977) Identification and nomenclature for G- and R-banded bovine chromosomes.Can J Genet Cytol 19: 271–282.
Mayr B, Czaker R (1981) Variable position of nucleolus organizer regions in Bovidae.Experientia 73: 563–565.
Perry P, Wolf S (1974) New Giemsa method for the differential staining of sister chromatids.Nature 257: 156–158.
Reading Conference (1980)Proceedings of the First International Conference for the Standardization of Banded Karyotypes of Domestic Animals. (Ford CE, Pollok DL, Gustavsson I, eds).Hereditas 92: 145–162.
Threadgill DW, Womack JE (1990) Genomic analysis of the major bovine milk protein genes.Nucleic Acids Res 18: 6935–6942.
Author information
Authors and Affiliations
Additional information
accepted for publication by H. C. Macgregor
Rights and permissions
About this article
Cite this article
Iannuzzi, L. G- and R-banded prometaphase karyotypes in cattle (Bos taurus L.). Chromosome Res 4, 448–456 (1996). https://doi.org/10.1007/BF02265052
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF02265052