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Determination of F1 hybrid seed purity in pepper using PCR-based markers

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Abstract

We present the potential use of two kinds of PCR-based markers, RAPDs (Random Amplified Polymorphic DNA) and SPARs (Single Primer Amplification Reactions), as tools for hybrid seed purity determination. Five F1 pepper hybrids (Capsicum annuum L.) and their parents were analyzed with 100 10-mer primers and 10 nucleotide repeat primers. We found at least one useful marker for testing the purity of all hybrids studied. Despite their dominant inheritance these markers could be an efficient implement in the process of quality testing of hybrid seeds.

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Authors and Affiliations

  1. Departament de Genètica Vegetal, Carretera de Cabrils, s/n., Institut de Recerca i Tecnologia Agroalimentàries (IRTA), 08348-Cabrils, Spain

    Jordi Ballester & M. Carmen de Vicente

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  1. Jordi Ballester
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  2. M. Carmen de Vicente
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Ballester, J., de Vicente, M.C. Determination of F1 hybrid seed purity in pepper using PCR-based markers. Euphytica 103, 223–226 (1998). https://doi.org/10.1023/A:1018372523343

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